Hu Z H, Broer R, Westerlaken J, Martens J W, Jin F, Jehle J A, Wang L M, Vlak J M
Department of Virology, Wageningen Agricultural University, Netherlands.
Virus Res. 1997 Jan;47(1):91-7. doi: 10.1016/s0168-1702(96)01406-2.
A putative ecdysteroid UDP-glucosyltransferase (egt) gene was identified in the single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria (BusuNPV). This is the first egt gene to be characterized in a SNPV, suggesting that egt genes are prevalent in nucleopolyhedroviruses and possibly in all baculoviruses. The open reading frame (ORF) of the gene is 1539 nucleotides (nt) long, encoding a putative protein (EGT) of 513 amino acids (aa) with a M of 58922. The 5' noncoding region contains three possible TATA boxes. A polyadenylation signal, AATAAA, was found downstream of the translation stop codon. A putative signal peptide of 16 residues was present at the N-terminus of the EGT. The BusuNPV egt gene has a high degree of nucleotide and amino acid sequence homology to multiple nucleocapsid (M) NPV egt genes, the highest being to the Spodoptera exigua MNPV egt. A phylogenetic tree of eleven known EGTs was constructed using maximum parsimony analysis.
在桑剑纹夜蛾单粒包埋核型多角体病毒（BusuNPV）中鉴定出一个假定的蜕皮甾类UDP-葡萄糖基转移酶（egt）基因。这是在单粒包埋核型多角体病毒中鉴定出的首个egt基因，表明egt基因在核型多角体病毒中普遍存在，可能在所有杆状病毒中也普遍存在。该基因的开放阅读框（ORF）长1539个核苷酸（nt），编码一个由513个氨基酸（aa）组成的假定蛋白质（EGT），分子量为58922。5'非编码区包含三个可能的TATA框。在翻译终止密码子下游发现了一个聚腺苷酸化信号AATAAA。EGT的N端存在一个由16个残基组成的假定信号肽。BusuNPV的egt基因与多个单粒包埋核型多角体病毒（M）NPV的egt基因具有高度的核苷酸和氨基酸序列同源性，与甜菜夜蛾MNPV的egt同源性最高。使用最大简约法构建了11种已知EGT的系统发育树。
