Hunt C R, Hale R J, Buckley C H, Hunt J
Department of Reproductive Pathology, St Mary's Hospital, Manchester.
J Clin Pathol. 1996 Dec;49(12):971-4. doi: 10.1136/jcp.49.12.971.
To assess overexpression of the proposed tumour suppressor gene product p53 using the mouse monoclonal antibody DO-7 in the three main subtypes of carcinoma of the uterine cervix and to evaluate its value as a prognostic indicator.
Eighty two cases of FIGO Stage IB/IIA uterine cervical carcinoma were studied retrospectively. The tumours had been previously typed into adenocarcinomas, squamous carcinomas and adenosquamous carcinomas after the tissue had been fixed in formalin and embedded in paraffin wax. p53 protein expression was assessed using a standard immunohistochemical technique and the findings were correlated with tumour type, lymph node status and clinical outcome.
In total, the p53 gene product was overexpressed in 17.1% (14/82) of all carcinomas and also in areas of cervical intraepithelial neoplasia grade III adjacent to invasive squamous carcinoma. Where present, the normal epithelium was uniformly negative. No association was found between p53 overexpression and tumour subtype, lymph node status or clinical outcome.
It seems unlikely that p53 analysis will be of value in determining prognosis in carcinoma of the uterine cervix.
使用小鼠单克隆抗体DO-7评估拟肿瘤抑制基因产物p53在子宫颈癌三种主要亚型中的过表达情况，并评估其作为预后指标的价值。
回顾性研究82例国际妇产科联盟（FIGO）分期为IB/IIA期的子宫颈癌病例。这些肿瘤在组织用福尔马林固定并石蜡包埋后，先前已被分类为腺癌、鳞癌和腺鳞癌。采用标准免疫组织化学技术评估p53蛋白表达，并将结果与肿瘤类型、淋巴结状态和临床结局相关联。
总体而言，p53基因产物在所有癌组织的17.1%（14/82）中过表达，在浸润性鳞癌旁的宫颈上皮内瘤变III级区域也过表达。正常上皮在存在的情况下均为阴性。未发现p53过表达与肿瘤亚型、淋巴结状态或临床结局之间存在关联。
p53分析在确定子宫颈癌预后方面似乎没有价值。
