Izzo A A, Benveniste J
INSERM U 200, Clamart, France.
J Pharmacol Toxicol Methods. 1996 Dec;36(4):219-21. doi: 10.1016/s1056-8719(96)00129-3.
After HPLC purification of human blood extracts, paf-acether (paf) was found exclusively as a lipoprotein-bound compound (lipopaf), whereas free-paf was absent. When the same samples (or lipopaf recovered from HPLC) were purified by TLC, both free-paf and lipopaf were detected. The free-paf detected in blood samples could thus result from lipopaf dissociation during TLC purification.
对人体血液提取物进行高效液相色谱（HPLC）纯化后，仅发现血小板活化因子（PAF）以脂蛋白结合化合物（脂蛋白结合型PAF）的形式存在，而未检测到游离型PAF。当对相同样本（或从HPLC回收的脂蛋白结合型PAF）进行薄层色谱（TLC）纯化时，游离型PAF和脂蛋白结合型PAF均被检测到。因此，血液样本中检测到的游离型PAF可能是TLC纯化过程中脂蛋白结合型PAF解离所致。
