Benveniste J, Nunez D, Duriez P, Korth R, Bidault J, Fruchart J C
INSERM U 200, Université Paris-Sud, Clamart, France.
FEBS Lett. 1988 Jan 4;226(2):371-6. doi: 10.1016/0014-5793(88)81456-x.
PAF-acether (PAF) is a newly formed mediator not normally present in circulating blood. A compound exhibiting all of its biological characteristics but coeluting with phosphatidylcholine (PC) in high-pressure liquid chromatography (HPLC) was unveiled ('peak X') in normal human plasma. A second HPLC run of peak X HPLC fractions revealed the presence of PAF itself with concomitant disappearance of peak X. Beside PAF, immunoreactive apolipoproteins A-I and E were found in peak X. Also lipoproteins (Ls) purified using either ultracentrifugation or immunoaffinity chromatography yielded peak X and, in a second HPLC run, authentic PAF. L-free plasma was devoid of peak X. Finally, after preincubation with plasma, labeled PAF was found associated with Ls. Thus in human blood preformed PAF is bound in high amounts to Ls, a result of interest given the role of Ls and platelets in vascular diseases and the present knowledge on PAF biosynthesis.
血小板活化因子（PAF）是一种新形成的介质，正常情况下不存在于循环血液中。在正常人血浆中发现了一种在高压液相色谱（HPLC）中与磷脂酰胆碱（PC）共洗脱但具有其所有生物学特性的化合物（“峰X”）。对峰X的HPLC馏分进行的第二次HPLC分析显示存在PAF本身，同时峰X消失。除了PAF，在峰X中还发现了免疫反应性载脂蛋白A-I和E。使用超速离心或免疫亲和色谱法纯化的脂蛋白（L）也产生了峰X，并且在第二次HPLC分析中产生了纯的PAF。无L血浆中没有峰X。最后，与血浆预孵育后，发现标记的PAF与L相关。因此，在人血液中，预先形成的PAF大量与L结合，鉴于L和血小板在血管疾病中的作用以及目前关于PAF生物合成的知识，这一结果值得关注。
