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盐诱导的酿酒酵母脂质组成变化及乙醇耐受性

Salt-induced changes in lipid composition and ethanol tolerance in Saccharomyces cerevisiae.

作者信息

Sharma S C, Raj D, Forouzandeh M, Bansal M P

机构信息

Department of Biochemistry, Panjab University Chandigarh, India.

出版信息

Appl Biochem Biotechnol. 1996 Feb;56(2):189-95. doi: 10.1007/BF02786949.

Abstract

The effect of salt stress on lipid composition and its relationship with ethanol tolerance in Saccharomyces cerevisiae was studied. Amounts of phospholipids as well as that of sterols decreased, whereas that of protein and glycolipids increased with increasing salt concentration. Relative proportion of amino phospholipids (phosphatidylethanolamine and phosphatidylserine) decreased, whereas that of phosphatidylcholine showed a reverse trend. Cells grown under increasing salt concentration were more resistant to ethanol-induced leakage of UV-absorbing substances, an index of ethanol endurance. Results showed an overlap between osmotolerance and ethanol tolerance in this strain.

摘要

研究了盐胁迫对酿酒酵母脂质组成的影响及其与乙醇耐受性的关系。随着盐浓度的增加,磷脂和甾醇的含量降低,而蛋白质和糖脂的含量增加。氨基磷脂(磷脂酰乙醇胺和磷脂酰丝氨酸)的相对比例降低,而磷脂酰胆碱的相对比例则呈现相反的趋势。在盐浓度增加的条件下生长的细胞对乙醇诱导的紫外线吸收物质泄漏更具抗性,这是乙醇耐受性的一个指标。结果表明该菌株的渗透耐受性和乙醇耐受性之间存在重叠。

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