Shinkai M, Yanase M, Honda H, Wakabayashi T, Yoshida J, Kobayashi T
Department of Biotechnology, Graduate School of Engineering, Nagoya University, Chikusa-ku.
Jpn J Cancer Res. 1996 Nov;87(11):1179-83. doi: 10.1111/j.1349-7006.1996.tb03129.x.
'Magnetite cationic liposomes (MCL)' were developed as a means to generate intracellular hyperthermia. Affinity of the MCL to glioma cells was ten times higher than that of magnetite 'neutral' liposomes due to the electrostatic interaction based on the positive charge of the MCL. Heat generation of the MCL was studied using agar phantoms and small pellets of rat glioma cells. When a high-frequency magnetic field, 118 kHz, 384 Oe was applied to glioma cells in the presence of MCL, the glioma cell pellet of 80 microl (5.4 mm in diameter) was heated to over 43 degrees C and all the cells died after 40 min irradiation owing to the hyperthermic effect. The terminal temperature of the cell pellet was proportional to the pellet volume when other parameters were constant. It thus appears that the MCL can heat a tumor of more than 80 microl in volume to above 42 degrees C.
“磁铁矿阳离子脂质体（MCL）”被开发出来作为一种产生细胞内热疗的手段。由于MCL带正电荷所产生的静电相互作用，MCL对胶质瘤细胞的亲和力比磁铁矿“中性”脂质体高10倍。使用琼脂模型和大鼠胶质瘤细胞小丸对MCL的发热情况进行了研究。当在存在MCL的情况下，对胶质瘤细胞施加118kHz、384奥斯特的高频磁场时，80微升（直径5.4毫米）的胶质瘤细胞小丸被加热到43摄氏度以上，并且在照射40分钟后所有细胞因热疗效应而死亡。当其他参数恒定时，细胞小丸的最终温度与小丸体积成正比。因此，似乎MCL能够将体积超过80微升的肿瘤加热到42摄氏度以上。
