Owen C S, Sykes N L
J Immunol Methods. 1984 Oct 12;73(1):41-8. doi: 10.1016/0022-1759(84)90029-2.
Magnetic labeling by a magnetite-antibody conjugate has been combined with magnetic filtration (high gradient magnetic separation) to effect a rapid and efficient separation of a selected cell population from a suspension of single cells. Samples of more than 10(8) cells could be fractionated in about 5 min with complete recovery. The system has been applied to a model system using red blood cells (sheep or chicken) and commercial antibodies against species-determined cell surface antigens. Enrichments of labeled cells by factors of up to 37-fold were observed. The approach was relatively insensitive to details in the experimental protocol and to the number of unlabeled cells which were in the sample. Thus, the method was easy to use and can readily be scaled up to handle large samples containing 10(8) labeled cells in a total of 10(11) or more. It should be useful as a pre-enrichment scheme for suspensions in which cells of interest are rare and, consequently, very inefficiently sorted by fluorescence-activated instruments.
通过磁铁矿 - 抗体缀合物进行的磁性标记已与磁过滤（高梯度磁分离）相结合，以实现从单细胞悬液中快速有效地分离选定的细胞群体。超过10⁸个细胞的样本可在约5分钟内分级分离，且完全回收。该系统已应用于一个模型系统，使用红细胞（绵羊或鸡）以及针对物种特异性细胞表面抗原的商业抗体。观察到标记细胞的富集倍数高达37倍。该方法对实验方案的细节以及样本中未标记细胞的数量相对不敏感。因此，该方法易于使用，并且可以很容易地扩大规模，以处理总共含有10¹¹个或更多细胞且其中有10⁸个标记细胞的大样本。对于感兴趣的细胞稀少、因此通过荧光激活仪器分选效率极低的悬液，它作为一种预富集方案应该是有用的。
