Bahr A, Möller-Rieker S, Hankeln T, Kraemer C, Protin U, Schmidt E R
Institut für Molekulargenetik, gentechnologische Sicherheitsforschung und Beratung, Universität Mainz, Germany.
Yeast. 1997 Feb;13(2):163-9. doi: 10.1002/(SICI)1097-0061(199702)13:2<163::AID-YEA54>3.0.CO;2-4.
The complete nucleotide sequence of a 39,090 bp segment from the left arm of yeast chromosome IV was determined. Twenty-one open reading frames (ORFs) longer than 100 amino acids and a Gly-tRNA gene were discovered. Nine of the 21 ORFs (D0892, D1022, D1037, D1045, D1057, D1204, D1209, D1214, D1219) correspond to the previously sequenced Saccharomyces cerevisiae genes for the NAD-dependent glutamate dehydrogenase (GDH), the secretory component (SHR3), the GABA transport protein (UGA4), the high mobility group-like protein (NHP2), the hydroxymethylbilane synthase (HEM3), the methylated DNA protein-cysteine S-methyltransferase (MGT1), a putative sugar transport protein, the Shm1 protein (SHM1) and the anti-silencing protein (ASF2). The inferred amino acid sequences of 11 ORFs show significant similarity with known proteins from various organisms, whereas the remaining ORF does not share any similarity with known proteins.
测定了酵母四号染色体左臂上一段39,090 bp片段的完整核苷酸序列。发现了21个长度超过100个氨基酸的开放阅读框（ORF）和一个甘氨酸转运RNA基因。21个ORF中的9个（D0892、D1022、D1037、D1045、D1057、D1204、D1209、D1214、D1219）对应于先前测序的酿酒酵母中与NAD依赖型谷氨酸脱氢酶（GDH）、分泌成分（SHR3）、GABA转运蛋白（UGA4）、高迁移率族样蛋白（NHP2）、羟甲基胆色素原合酶（HEM3）、甲基化DNA蛋白-半胱氨酸S-甲基转移酶（MGT1）、一种假定的糖转运蛋白、Shm1蛋白（SHM1）和抗沉默蛋白（ASF2）相关的基因。11个ORF的推断氨基酸序列与来自各种生物体的已知蛋白质具有显著相似性，而其余的ORF与已知蛋白质没有任何相似性。
