来自酿酒酵母四号染色体左臂上一个36.7 kb片段的序列揭示了20个不重叠的开放阅读框（ORF），包括SIT4、FAD1、NAM1、RNA11、SIR2、NAT1、PRP9、ACT2和MPS1以及11个新的ORF。
The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs.
作者信息
Sarén A M, Laamanen P, Lejarcegui J B, Paulin L
机构信息
DNA Synthesis and Sequencing Laboratory, Institute of Biotechnology, University of Helsinki, Finland.
出版信息
Yeast. 1997 Jan;13(1):65-71. doi: 10.1002/(SICI)1097-0061(199701)13:1<65::AID-YEA50>3.0.CO;2-T.
A 36,688 bp fragment from the left arm of chromosome IV of saccharomyces cerevisiae was sequenced. Sequence analysis identified 20 complete non-overlapping open reading frames (ORFs) of at least 100 amino acids. Nine of these correspond to previously identified and sequenced genes: SIT4/PH1, FAD1, NAM1/MTF2, RNA11, SIR2/MAR1, NAT1/AAA1, PRP9, ACT2 and MPS1/RPK1. Three ORFs show homology to previously sequenced genes. One ORF exhibits a hypothetical yabO/yceC/YfiI family signature and one has the ATP-dependent helicase signature of the DEAD and DEAH box families. Six ORFs show no appreciable homology to any proteins in the database. One of these is identical to yeast expressed sequence tags and therefore corresponds to and expressed gene. In addition, two partial ORFs and 11 ORFs that are totally internal and are not likely to be functional were detected.
对酿酒酵母第四条染色体左臂上一段36,688 bp的片段进行了测序。序列分析确定了20个完整的、不重叠的、至少含100个氨基酸的开放阅读框（ORF）。其中9个与先前鉴定和测序的基因相对应：SIT4/PH1、FAD1、NAM1/MTF2、RNA11、SIR2/MAR1、NAT1/AAA1、PRP9、ACT2和MPS1/RPK1。3个ORF与先前测序的基因具有同源性。1个ORF具有假定的yabO/yceC/YfiI家族特征，1个具有DEAD和DEAH框家族的ATP依赖性解旋酶特征。6个ORF与数据库中的任何蛋白质均无明显同源性。其中1个与酵母表达序列标签相同，因此对应一个表达基因。此外，还检测到2个部分ORF和11个完全位于内部且可能无功能的ORF。
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