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对酵母第十五号染色体右臂上一个36.2 kb片段的测序与分析揭示了19个开放阅读框,包括SNF2(5'端)、CPA1、SLY41、一个假定的转运ATP酶、一个假定的核糖体蛋白以及一个SNF2同源物。

Sequence and analysis of a 36.2 kb fragment from the right arm of yeast chromosome XV reveals 19 open reading frames including SNF2 (5' end), CPA1, SLY41, a putative transport ATPase, a putative ribosomal protein and an SNF2 homologue.

作者信息

Poirey R, Cziepluch C, Tobiasch E, Pujol A, Kordes E, Jauniaux J C

机构信息

Angewandte Tumorvirologie, Abteilung 0610, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

出版信息

Yeast. 1997 Apr;13(5):479-82. doi: 10.1002/(SICI)1097-0061(199704)13:5<479::AID-YEA104>3.0.CO;2-G.

Abstract

The complete sequence of a 36 196 bp DNA segment located on the right arm of chromosome XV of Saccharomyces cerevisiae has been determined and analysed. The sequence includes the 5' coding region of the SNF2 gene, the CPA1 leader peptide sequence and 17 open reading frames (ORFs) of at least 100 amino acids. Two of these correspond to previously known genes (CPA1, SLY41), whereas 15 correspond to new genes. The putative translation products of three ORFs show significant similarity with known proteins: one is a putative transport ATPase, another appears to be a ribosomal protein, and the third is an Snf2p homologue.

摘要

已确定并分析了位于酿酒酵母第十五号染色体右臂上一段36196碱基对DNA片段的完整序列。该序列包括SNF2基因的5'编码区、CPA1前导肽序列以及17个至少含100个氨基酸的开放阅读框(ORF)。其中两个与先前已知基因(CPA1、SLY41)相对应,而另外15个对应新基因。三个开放阅读框的推定翻译产物与已知蛋白质显示出显著相似性:一个是推定的转运ATP酶,另一个似乎是核糖体蛋白,第三个是Snf2p同源物。

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