Park H S, Nahm D H
Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
Ann Allergy Asthma Immunol. 1997 Feb;78(2):225-9. doi: 10.1016/s1081-1206(10)63392-3.
Serratial peptidase and lysozyme are often used as anti-inflammatory agents. There have been very few documented cases of occupational allergy caused by these substances. We report a case of a pharmaceutical industry worker who developed occupational asthma and rhinitis caused by both serratial peptidase and lysozyme chloride.
It is important to alert physicians to the possibility of occupational asthma when dealing with workers in the pharmaceutical industry.
The patient had strong positive responses to peptidase and lysozyme extracts on skin-prick tests. Bronchoprovocation tests showed a dual asthmatic response to peptidase and an early asthmatic response to lysozyme. Serum specific IgE antibodies to peptidase and lysozyme were detected by enzyme-linked immunosorbent assay (ELISA). In order to further characterize the allergenic component of these extracts, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotting studies were also performed. More than ten components ranging form 7.3 to 83.1 kD were found in peptidase extracts, and two IgE binding components (67, 10.9 kD) were detected within the lysozyme extracts.
These findings suggest that inhalation of peptidase and lysozyme can induce IgE-mediated bronchoconstrictions in an exposed worker.
沙雷氏菌蛋白酶和溶菌酶常被用作抗炎剂。由这些物质引起职业性过敏的记录病例非常少。我们报告一例制药行业工人病例，其因沙雷氏菌蛋白酶和氯化溶菌酶而患上职业性哮喘和鼻炎。
在诊治制药行业工人时，提醒医生注意职业性哮喘的可能性很重要。
患者皮肤点刺试验对蛋白酶和溶菌酶提取物有强阳性反应。支气管激发试验显示对蛋白酶有双重哮喘反应，对溶菌酶有早期哮喘反应。通过酶联免疫吸附测定（ELISA）检测到针对蛋白酶和溶菌酶的血清特异性IgE抗体。为进一步鉴定这些提取物的致敏成分，还进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和电印迹研究。在蛋白酶提取物中发现十多种分子量从7.3至83.1 kD的成分，在溶菌酶提取物中检测到两种IgE结合成分（67、10.9 kD）。
这些发现表明，吸入蛋白酶和溶菌酶可在接触的工人中诱发IgE介导的支气管收缩。
