Kim H Y, Nahm D H, Park H S, Choi D C
Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Kyung-Ki-Do, Korea.
Ann Allergy Asthma Immunol. 1999 Feb;82(2):174-8. doi: 10.1016/S1081-1206(10)62593-8.
Although there have been a few reports of occupational asthma due to cellulase in several occupational settings, this is the first case of cellulase-induced occupational asthma in an employee working in the textile industry. Its pathogenetic mechanism remains to be further clarified.
It is important to alert physicians to the possibility of occupational asthma caused by cellulase in workers of the textile industry.
The patient had atopy and strong positive responses to cellulase extract on skin prick tests. Bronchoprovocation test showed an early asthmatic response to cellulase extract. Serum specific IgE and specific IgG4 antibodies to cellulase were detected by enzyme-linked immunosorbent assay (ELISA). In order to further characterize the allergenic component of the extract, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotting studies were performed. Eight IgE binding components ranging from 6 to 97.5 kD were detected within the cellulase extract.
These findings suggest that inhalation of cellulase can induce IgE-mediated bronchoconstrictions in employees working in the textile industry.
尽管在一些职业环境中已有少数关于纤维素酶引起职业性哮喘的报道，但这是纺织行业一名员工因纤维素酶诱发职业性哮喘的首例病例。其发病机制仍有待进一步阐明。
提醒医生注意纺织行业工人中由纤维素酶引起职业性哮喘的可能性很重要。
该患者有特应性，皮肤点刺试验对纤维素酶提取物有强阳性反应。支气管激发试验显示对纤维素酶提取物有早期哮喘反应。通过酶联免疫吸附测定（ELISA）检测到血清中针对纤维素酶的特异性IgE和特异性IgG4抗体。为了进一步鉴定提取物的致敏成分，进行了十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和电印迹研究。在纤维素酶提取物中检测到8种分子量在6至97.5 kD之间的IgE结合成分。
这些发现表明，吸入纤维素酶可在纺织行业员工中诱发IgE介导的支气管收缩。
