McCain W C, Lee R, Johnson M S, Whaley J E, Ferguson J W, Beall P, Leach G
Oak Ridge Institute for Science and Education, Tennessee, USA.
J Toxicol Environ Health. 1997 Feb 7;50(2):113-24. doi: 10.1080/009841097160528.
This study investigated the lethal interaction of pyridostigmine bromide (PB), permethrin, and DEET when given to adult male rats by gavage and was separated into two phases. Phase I determined the acute oral lethal dose-response relationship of each compound with the vehicle, propylene glycol. Phase II was divided into two portions: a dose-response study using probit units obtained from phase I [lethal dose (LD) 16, 30, 50, 70, and 84], and an interaction study that contained low levels (calculated LD16, additive LD32) of the two compounds while the concentration of the third compound was varied. Rats were fasted overnight, dosed, and observed for 14 d. A significant increase in lethality occurred when PB, permethrin, and DEET were given concurrently when compared to expected additive values. Furthermore, solutions containing PB and permethrin or PB and DEET also caused a significant increase in lethality when compared to expected additive values. This information suggests that lethality in this study was more than an additive effect.
本研究通过灌胃给予成年雄性大鼠溴吡斯的明（PB）、氯菊酯和避蚊胺，研究它们的致死相互作用，并分为两个阶段。第一阶段确定了每种化合物与赋形剂丙二醇的急性经口致死剂量-反应关系。第二阶段分为两部分：使用从第一阶段获得的概率单位进行剂量-反应研究[致死剂量（LD）16、30、50、70和84]，以及一项相互作用研究，其中包含两种化合物的低水平（计算得出的LD16、相加LD32），同时改变第三种化合物的浓度。大鼠禁食过夜，给药，并观察14天。与预期相加值相比，同时给予PB、氯菊酯和避蚊胺时致死率显著增加。此外，与预期相加值相比，含有PB和氯菊酯或PB和避蚊胺的溶液也导致致死率显著增加。该信息表明本研究中的致死率不仅仅是相加效应。
