ADA Technologies, Inc., 8100 Shaffer Parkway, Littleton, Colorado 80127, USA.
J Proteome Res. 2009 Nov;8(11):5031-40. doi: 10.1021/pr900452s.
We reported here a novel, ready-to-use bioarray platform and methodology for construction of sensitive carbohydrate cluster microarrays. This technology utilizes a three-dimensional (3-D) poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal aminooxy and hydrazide groups for site-specific coupling of carbohydrates. A wide range of saccharides, including monosaccharides, oligosaccharides and polysaccharides of diverse structures, are applicable for the 3-D bioarray platform without prior chemical derivatization. The process of carbohydrate coupling is effectively accelerated by microwave radiation energy. The carbohydrate concentration required for microarray fabrication is substantially reduced using this technology. Importantly, this bioarray platform presents sugar chains in defined orientation and cluster configurations. It is, thus, uniquely useful for exploration of the structural and conformational diversities of glyco-epitope and their functional properties.
我们在这里报道了一种新颖的、即用型的生物芯片平台和方法，用于构建敏感的糖簇微阵列。该技术利用了组装在玻璃表面上的三维（3-D）聚酰胺胺星型树枝状大分子单层，其末端具有氨氧基和酰肼基团，可用于糖的特异性偶联。广泛的糖，包括单糖、寡糖和结构多样的多糖，适用于无需事先化学衍生化的 3-D 生物芯片平台。糖偶联过程通过微波辐射能量得到有效加速。使用这项技术，微阵列制造所需的糖浓度大大降低。重要的是，这个生物芯片平台以确定的方向和簇结构呈现糖链。因此，它对于探索糖基表位的结构和构象多样性及其功能特性非常有用。
