Amoah E A, Gelaye S
Agricultural Research Station, Fort Valley State University, GA 31030-3298, USA.
J Anim Sci. 1997 Feb;75(2):578-85. doi: 10.2527/1997.752578x.
Goat selection and reproduction have resulted from using conventional methods of natural mating and artificial insemination. Genetic improvements resulting from these are usually slow. Innovative developments in biotechnology rapidly propagate superior genes, offering hope for modeling and designing animals to fit market and environmental requirements. Use of Tris, citric acid, fructose, egg yolk, and glycerol extender has enabled goat sperm to be stored successfully for several years before being used in cervical or laparoscopic insemination. Laparoscopic recovery of goat embryos to reduce adhesions from repeat surgeries has great potential in improving embryo production for direct transfer or for future transfer after cryopreservation. Goat kids have been produced, as a result of experiments to refine techniques of in vitro maturation and fertilization of recovered oocytes, with successful culture and transfer of embryos. In vitro fertilization technology is also essential for cloning goat embryos and for gene transfer. Transgenic goats have already been produced due to new genes being expressed from biologically active molecules altering the phenotype of the transferred goat. The introduced gene is capable of transmission between generations. The goat's diversified commercial value and convenient size make it a benefactor to new technology for rapid genetic improvement as a supplement to conventional selection methods.
山羊的选育和繁殖一直采用自然交配和人工授精等传统方法。由此带来的遗传改良通常较为缓慢。生物技术的创新发展能够快速传播优良基因,为塑造和设计符合市场及环境需求的动物带来了希望。使用含有三羟甲基氨基甲烷、柠檬酸、果糖、蛋黄和甘油的稀释液,已使山羊精子能够成功保存数年,之后用于子宫颈或腹腔镜授精。腹腔镜采集山羊胚胎以减少重复手术造成的粘连,这在提高用于直接移植或冷冻保存后未来移植的胚胎产量方面具有巨大潜力。通过完善回收卵母细胞的体外成熟和受精技术的实验,已成功培养并移植胚胎,从而培育出了山羊幼崽。体外受精技术对于克隆山羊胚胎和基因转移也至关重要。由于生物活性分子表达的新基因改变了转基因山羊的表型,转基因山羊已经培育成功。导入的基因能够在代际间传递。山羊具有多样的商业价值且体型适宜,这使其成为新技术快速进行遗传改良的受益者,可作为传统选育方法的补充。