Xu Bingbing, Wang Ruijun, Wang Zhiying, Liu Hongfu, Wang Zhen, Zhang Weihang, Zhang Yanjun, Su Rui, Liu Zhihong, Liu Yongbin, Li Jinquan, Zhang Jiaxin
College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction, Hohhot, China.
Front Vet Sci. 2022 Oct 20;9:1004683. doi: 10.3389/fvets.2022.1004683. eCollection 2022.
The current study aimed to detect the relationship between the spermatozoa cryotolerance and the post-thawed sperm lipidome. Ejaculates from 20 goats, and performed a uniform frozen-thawed procedure in this study. According to the total motility of thawed sperm of goats, semen samples were classified into HF group (High Freezers, = 8) with >60% total motility and LF group (Low Freezers, = 8) with < 45% total motility. The lipidomic analysis based on UHPLC-MS/MS was utilized to investigate the relationship between sperm cryotolerance and their lipid metabolites expression. The results showed that the cryotolerance of sperm from different individual goats were in great variation. The total motility of post-thawed sperm in HF group (60.93 ± 2.43%) is significantly higher than that in LF group (34.04 ± 3.41%, < 0.01). And the post-thawed sperm in HF group exhibited significantly higher plasma membrane (59.06 ± 2.34%) and acrosome integrity (62.93 ± 1.15%) than that in LF group (34.06 ± 4.85%, 44.92 ± 2.19% respectively, < 0.01). The total of 29 lipid subclasses and 1,133 lipid molecules in the post-thawed goat sperm were identified by lipidomics analysis. The lipid content of thawed sperm in HF group was higher than that in LF group, the lipid profile in HF group was significantly separated from LF group, which indicated that the difference in lipid composition and lipid metabolism mode of sperm between the two groups was existed, especially the expression of phosphatidylcholine and triglyceride molecules. In conclusion, the cryotolerance of sperm from different individual goats were in great variation. Sperm with high cryotolerance may be able to uptake more lipids during cryopreservation. The increase in phosphatidylcholine and triglyceride content of thawed. Sperm may relate to more active lipid anabolic processes.
本研究旨在检测精子冷冻耐受性与解冻后精子脂质组之间的关系。本研究采集了20只山羊的精液，并进行了统一的冷冻解冻程序。根据解冻后山羊精子的总活力，将精液样本分为高活力组（HF组，n = 8），总活力>60%，以及低活力组（LF组，n = 8），总活力<45%。采用基于超高效液相色谱-串联质谱（UHPLC-MS/MS）的脂质组学分析方法，研究精子冷冻耐受性与其脂质代谢产物表达之间的关系。结果表明，不同个体山羊精子的冷冻耐受性差异很大。HF组解冻后精子的总活力（60.93±2.43%）显著高于LF组（34.04±3.41%，P<0.01）。HF组解冻后精子的质膜完整性（59.06±2.34%）和顶体完整性（62.93±1.15%）显著高于LF组（分别为34.06±4.85%、44.92±2.19%，P<0.01）。通过脂质组学分析鉴定了解冻后山羊精子中的29种脂质亚类和1133种脂质分子。HF组解冻后精子的脂质含量高于LF组，HF组的脂质谱与LF组明显分离，这表明两组精子的脂质组成和脂质代谢模式存在差异，尤其是磷脂酰胆碱和甘油三酯分子的表达。总之，不同个体山羊精子的冷冻耐受性差异很大。冷冻耐受性高的精子在冷冻保存过程中可能能够摄取更多的脂质。解冻后精子中磷脂酰胆碱和甘油三酯含量的增加可能与更活跃的脂质合成代谢过程有关。
