Marian M, Parrino C, Leo A M, Vincenti E, Bindoli A, Scutari G
Department of Biology Chemistry, University of Padova, Italy.
Neurochem Res. 1997 Mar;22(3):287-92. doi: 10.1023/a:1022438805337.
The sensitivity of the mitochondrial energy production system to propofol (DPP) has been investigated in rat brain synaptosomes. DPP at 0.8 mM concentration produced a partial inhibition of coupled respiration, an apparent decrease of the oxygen uptake stimulation induced by CCCP and a full inhibition of the mitochondrial ATP production by synaptosomes. Higher concentrations of DPP (1 mM) fully abolish uncoupler-dependent stimulation and at 1.3 mM DPP also coupled respiration is completely blocked. Similar results were obtained when dinitrophenol replaced CCCP and phenol or propylbenzene replaced DPP. The presence of the alkyl residues seems critical for the DPP effect. In the presence of 30 mM glutamate both respiration and ATP production are enhanced but DPP effects are similar to those obtained in the absence of glutamate.
已在大鼠脑突触体中研究了线粒体能量产生系统对丙泊酚（DPP）的敏感性。0.8 mM浓度的DPP对偶联呼吸产生部分抑制，对CCCP诱导的氧摄取刺激有明显降低作用，并完全抑制突触体的线粒体ATP产生。更高浓度的DPP（1 mM）完全消除了解偶联剂依赖性刺激，在1.3 mM DPP时，偶联呼吸也被完全阻断。当二硝基苯酚替代CCCP且苯酚或丙基苯替代DPP时，获得了类似的结果。烷基残基的存在似乎对DPP的作用至关重要。在存在30 mM谷氨酸的情况下，呼吸和ATP产生均增强，但DPP的作用与在不存在谷氨酸时获得的作用相似。
