Karttunen R A, Karttunen T J, Yousfi M M, el-Zimaity H M, Graham D Y, el-Zaatari F A
Dept. of Medicine, and Inflammatory Bowel Disease Laboratory, Veterans Affairs Medical Center, Houston, TX 77030, USA.
Scand J Gastroenterol. 1997 Jan;32(1):22-7. doi: 10.3109/00365529709025058.
We studied the mRNA expressions of interferon-gamma (IFN-gamma), interleukin-10 (IL-10), and IL-12 in gastric biopsy and blood samples from patients with and without Helicobacter pylori infection, by reverse-transcription polymerase chain reaction (RT-PCR).
RT-PCR was performed on total RNA preparations, and the expressed mRNA were semiquantitated on the basis of band intensities on Southern blots.
In gastric mucosa the expression of IFN-gamma and IL-10 was found in most patients with and without H. pylori infection, whereas IL-12 was found in most of the infected ones. The level of IFN-gamma and IL-10 did not differ between groups, whereas the IL-12 level was significantly higher in those with H. pylori infection. In the blood IFN-gamma expression was found in most samples, with higher level in patients with gastritis than in normals. Few blood samples (33%) had IL-12, and none had IL-10.
IFN-gamma and IL-10 expressions in healthy mucosa may indicate a biologic role in a healthy state. IL-12 expression in mucosa was related to the presence of bacterial stimulant and therefore resembles proinflammatory cytokines.
我们通过逆转录聚合酶链反应（RT-PCR）研究了幽门螺杆菌感染患者与未感染患者胃活检及血液样本中γ-干扰素（IFN-γ）、白细胞介素-10（IL-10）和IL-12的mRNA表达情况。
对总RNA制剂进行RT-PCR，并根据Southern印迹上条带强度对表达的mRNA进行半定量分析。
在胃黏膜中，大多数幽门螺杆菌感染患者与未感染患者均发现有IFN-γ和IL-10表达，而大多数感染患者发现有IL-12表达。两组间IFN-γ和IL-10水平无差异，而幽门螺杆菌感染患者的IL-12水平显著更高。在血液中，大多数样本发现有IFN-γ表达，胃炎患者的水平高于正常人群。少数血液样本（33%）有IL-12表达，无一例有IL-10表达。
健康黏膜中IFN-γ和IL-10表达可能表明其在健康状态下具有生物学作用。黏膜中IL-12表达与细菌刺激物的存在有关，因此类似于促炎细胞因子。
