Safikhani Mahmoodzadeh Abdollah, Moazamian Elham, Shamsdin Seyedeh Azra, Kaydani Gholam Abas
Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Gasteroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Middle East J Dig Dis. 2024 Oct;16(4):235-241. doi: 10.34172/mejdd.2024.398. Epub 2024 Oct 30.
is a gram-negative pathogen. The infection caused by this pathogen may result in gastritis and can increase the risk of gastric cancer. This study investigated the relationship between infection as the main risk factor for gastritis and changes in serum inflammatory cytokine levels.
Blood samples from 85 patients with stomach pain, including 46 -positive (Hp) and 39 H. pylori-negative (Hp) cases, were collected and referred to a gastroenterologist. After isolation and identification of , the severity of gastritis was determined for each patient based on the histopathological findings. Finally, the serum levels of cytokines were measured using the multiplex kit and flow cytometry methods.
There were significant differences in the levels of interleukin-2 (IL-2), IL-4, IL-17A, IL-17F, IL-22, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) between the Hp and the Hp specimens (≤0.05). The levels of IL-2, IL-17A, IL-17F, IL-22, TNF-α, and IFN-γ were significantly higher in patients with mild and moderate gastritis than Hp group (≤0.05). In addition, IL-4 significantly increased in patients with moderate gastritis compared with Hp individuals (=0.008).
Among the inflammatory cytokines evaluated in this study, IL-17A, IL-17F, and IL-22 may play a crucial role in developing moderate gastritis in infected patients with .
是一种革兰氏阴性病原体。该病原体引起的感染可能导致胃炎，并会增加患胃癌的风险。本研究调查了作为胃炎主要危险因素的感染与血清炎症细胞因子水平变化之间的关系。
收集了85例胃痛患者的血样，其中46例幽门螺杆菌（Hp）阳性和39例Hp阴性病例，并转诊给胃肠病学家。在分离和鉴定后，根据组织病理学结果确定每位患者的胃炎严重程度。最后，使用多重试剂盒和流式细胞术方法测量细胞因子的血清水平。
Hp阳性和Hp阴性样本之间白细胞介素-2（IL-2）、IL-4、IL-17A、IL-17F、IL-22、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平存在显著差异（P≤0.05）。轻度和中度胃炎患者的IL-2、IL-17A、IL-17F、IL-22、TNF-α和IFN-γ水平显著高于Hp阴性组（P≤0.05）。此外，与Hp阴性个体相比，中度胃炎患者的IL-4显著升高（P=0.008）。
在本研究评估的炎症细胞因子中，IL-17A、IL-17F和IL-22可能在感染患者发生中度胃炎中起关键作用。
