Cozzi R, Ricordy R, Aglitti T, Gatta V, Perticone P, De Salvia R
Dipartimento di Biologia, Università degli Studi Roma Tre, Italy.
Carcinogenesis. 1997 Jan;18(1):223-8. doi: 10.1093/carcin/18.1.223.
Naturally occurring antioxidants are extensively studied for their capacity to protect organisms and cells from damage induced by oxygen reactive species. In fact, oxidative stress is considered a cause of aging, degenerative disease and cancer. We have focused our attention on two agents, ascorbic acid and beta-carotene, commonly considered to be antioxidants, but whose protective activity against cancer is insufficiently known. This paper reports on the ability of these agents to act against damage induced by H2O2 and bleomycin, in Chinese hamster ovary cells cultivated in vitro. Cytogenetic and cytofluorimetric analyses were performed. Both vitamins proved effective in reducing H2O2-induced sister chromatid exchanges, but increased H2O2- and bleomycin-induced chromosomal aberrations. Cytofluorimetric data, in contrast, showed that ascorbic acid and beta-carotene act as scavengers of endogeneous and H2O2-induced oxygen species.
天然存在的抗氧化剂因其保护生物体和细胞免受氧活性物质诱导损伤的能力而得到广泛研究。事实上，氧化应激被认为是衰老、退行性疾病和癌症的一个原因。我们将注意力集中在两种物质上，即抗坏血酸和β-胡萝卜素，它们通常被认为是抗氧化剂，但其对癌症的保护活性尚不清楚。本文报道了这些物质在体外培养的中国仓鼠卵巢细胞中对抗过氧化氢（H2O2）和博来霉素诱导损伤的能力。进行了细胞遗传学和细胞荧光分析。两种维生素都被证明能有效减少H2O2诱导的姐妹染色单体交换，但会增加H2O2和博来霉素诱导的染色体畸变。相比之下，细胞荧光分析数据表明，抗坏血酸和β-胡萝卜素可作为内源性和H2O2诱导的氧物种的清除剂。
