Flint J, Thomas K, Micklem G, Raynham H, Clark K, Doggett N A, King A, Higgs D R
MRC Molecular Haematology Unit, John Radcliffe Hospital, Headington, Oxford, UK.
Nat Genet. 1997 Mar;15(3):252-7. doi: 10.1038/ng0397-252.
We have sequenced a contiguous 284,495-bp segment of DNA extending from the terminal (TTAGGG)n repeats of the short arm of chromosome 16, providing a full description of the transition from telomeric through subtelomeric DNA to sequences that are unique to the chromosome. To complement and extend analysis of the primary sequence, we have characterized mRNA transcripts, patterns of DNA methylation and DNase I sensitivity. Together with previous data these studies describe in detail the structural and functional organization of a human telomeric region.
我们对一段连续的284,495碱基对的DNA片段进行了测序，该片段从16号染色体短臂的末端（TTAGGG）n重复序列开始延伸，完整描述了从端粒DNA经亚端粒DNA到该染色体特有序列的转变。为补充和拓展对一级序列的分析，我们对mRNA转录本、DNA甲基化模式及DNase I敏感性进行了特征分析。这些研究与先前的数据一起，详细描述了人类端粒区域的结构和功能组织。
