酿酒酵母中两个高亲和力硫酸盐转运蛋白的分子特征
Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae.
作者信息
Cherest H, Davidian J C, Thomas D, Benes V, Ansorge W, Surdin-Kerjan Y
机构信息
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
出版信息
Genetics. 1997 Mar;145(3):627-35. doi: 10.1093/genetics/145.3.627.
Abstract
Strains resistant to the toxic analogues of sulfate, selenate and chromate have been isolated. Their genetic analysis allowed us to identify four genes. One, called MET28, encodes a transcriptional factor. The three other genes, called SUL1, SUL2 and SUL3, encode proteins involved in sulfate transport. The sequence of Sul1p and Sul2p indicate that they are integral membrane proteins exhibiting, respectively, 11 and 10 transmembrane domains. Moreover, Sul1p and Sul2p share a high degree of similarity. Sulfate transport kinetic studies made with parental and mutant strains show that, as expected from genetic results, Saccharomyces cerevisiae has two high affinity sulfate transport systems. Sul3p has been shown to be involved in the transcriptional regulation of the SUL2 gene.
摘要
已分离出对硫酸盐、硒酸盐和铬酸盐的毒性类似物具有抗性的菌株。对它们的基因分析使我们能够鉴定出四个基因。其中一个名为MET28，编码一种转录因子。另外三个基因，名为SUL1、SUL2和SUL3，编码参与硫酸盐转运的蛋白质。Sul1p和Sul2p的序列表明它们是整合膜蛋白，分别具有11个和10个跨膜结构域。此外，Sul1p和Sul2p具有高度相似性。用亲本菌株和突变菌株进行的硫酸盐转运动力学研究表明，正如遗传结果所预期的那样，酿酒酵母有两个高亲和力硫酸盐转运系统。已证明Sul3p参与SUL2基因的转录调控。
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