Thielitz A, Helmdach M, Röpke E M, Gollnick H
Department of Dermatology and Venereology, Medical Faculty, Otto von Guericke University Magdeburg, Leipziger Strasse 44, D-39120 Magdeburg, Germany.
Br J Dermatol. 2001 Jul;145(1):19-27. doi: 10.1046/j.1365-2133.2001.04276.x.
The cyanoacrylate follicular biopsy is an established method for the examination of the horny layer and quantitative assessment of microcomedones. We have optimized the method by separating follicular casts mechanically from the cyanoacrylate strips.
To use this method to analyse topical therapy-induced changes of the lipid composition in the sebaceous follicular infundibulum.
Both the follicular casts and the residual skin surface strip, the last representing a mixture of stratum corneum and surface lipids, were extracted twice with n-hexane-ethanol under ultrasonication, evaporated, redissolved in chloroform-methanol and separated by high-performance thin layer chromatography, using cholesterol sulphate, cerebroside, ceramide types 3 and 4, cholesterol, oleic acid, triolein, cholesterol oleate and squalene as standards. Identification was performed by computer-assisted densitometric analysis. Six patient groups receiving adapalene 0.1%, tretinoin 0.025%, clindamycin 1%, clindamycin 1% + tretinoin 0.025%, benzoyl peroxide 5% or benzoyl peroxide 5% + erythromycin 2% were investigated before and 12 weeks after application.
A significant decrease in free fatty acid proportions combined with an increase in triglycerides was observed in the groups receiving antimicrobial therapy, supporting the hypothesis of lipolysis due to microbial colonization. The groups treated with topical retinoids showed an additional significant increase in ceramide subfractions, most probably reflecting their influence on epidermal keratinization.
Our method proved suitable for the detection of quantitative and qualitative changes in lipid profiles of both infundibulum cast content and surface lipids. It enabled simple, non-invasive and objective assessment of the most relevant lipid classes in the sebaceous infundibulum, and efficient monitoring of drug effects on the follicular infundibulum.
氰基丙烯酸酯毛囊活检是一种用于检查角质层和微粉刺定量评估的既定方法。我们通过从氰基丙烯酸酯条带中机械分离毛囊铸型优化了该方法。
使用该方法分析局部治疗引起的皮脂腺毛囊漏斗部脂质成分变化。
毛囊铸型和残留的皮肤表面条带（后者代表角质层和表面脂质的混合物）在超声作用下用正己烷 - 乙醇萃取两次，蒸发，再溶解于氯仿 - 甲醇中，并通过高效薄层色谱分离，使用硫酸胆固醇、脑苷脂、3型和4型神经酰胺、胆固醇、油酸、三油精、胆固醇油酸酯和角鲨烯作为标准品。通过计算机辅助光密度分析进行鉴定。对接受0.1%阿达帕林、0.025%维甲酸、1%克林霉素、1%克林霉素 + 0.025%维甲酸、5%过氧化苯甲酰或5%过氧化苯甲酰 + 2%红霉素的六个患者组在用药前和用药12周后进行了研究。
在接受抗菌治疗的组中观察到游离脂肪酸比例显著降低，同时甘油三酯增加，支持了微生物定植导致脂解的假说。局部用维甲酸治疗的组神经酰胺亚组分也显著增加，很可能反映了它们对表皮角质化的影响。
我们的方法证明适用于检测漏斗部铸型内容物和表面脂质的脂质谱定量和定性变化。它能够对皮脂腺漏斗部最相关的脂质类别进行简单、非侵入性和客观的评估，并有效监测药物对毛囊漏斗部的作用。
