分子信标：一种基于荧光的溶液杂交技术用于瘤胃细菌生态学研究的试验
Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.
作者信息
Schofield P, Pell A N, Krause D O
机构信息
Department of Animal Science, Cornell University, Ithaca, New York 14853, USA.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):1143-7. doi: 10.1128/aem.63.3.1143-1147.1997.
Abstract
Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.
摘要
分子信标是为溶液而非膜杂交开发的荧光探针。我们研究了这些探针在瘤胃微生物生态学研究中的实用性。测试了两种纤维素分解菌，即白色瘤胃球菌和琥珀酸纤维杆菌。在白色瘤胃球菌8和琥珀酸纤维杆菌S85共培养物的竞争实验中，膜杂交和溶液杂交给出了相似的结果。
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本文引用的文献
1
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Appl Environ Microbiol. 1978 Jan;35(1):156-65. doi: 10.1128/aem.35.1.156-165.1978.
2
The anaerobic mesophilic cellulolytic bacteria.厌氧嗜温性纤维素分解菌。
Bacteriol Rev. 1950 Mar;14(1):1-49. doi: 10.1128/br.14.1.1-49.1950.
3
Molecular beacons: probes that fluoresce upon hybridization.分子信标：杂交时发出荧光的探针。
Nat Biotechnol. 1996 Mar;14(3):303-8. doi: 10.1038/nbt0396-303.
4
An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.一种用于评估专性氨基酸发酵细菌在瘤胃氨基酸脱氨作用中角色的rRNA方法。
Appl Environ Microbiol. 1996 Mar;62(3):815-21. doi: 10.1128/aem.62.3.815-821.1996.
5
Utilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria.三种主要瘤胃纤维素分解菌对单个纤维糊精的利用情况
Appl Environ Microbiol. 1996 Mar;62(3):1084-8. doi: 10.1128/aem.62.3.1084-1088.1996.
6
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Appl Environ Microbiol. 1995 Apr;61(4):1348-51. doi: 10.1128/aem.61.4.1348-1351.1995.
7
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8
Commentary on the Hungate technique for culture of anaerobic bacteria.关于厌氧细菌培养的亨盖特技术的述评
Am J Clin Nutr. 1972 Dec;25(12):1324-8. doi: 10.1093/ajcn/25.12.1324.
9
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Appl Environ Microbiol. 1988 May;54(5):1079-84. doi: 10.1128/aem.54.5.1079-1084.1988.
10
DNA probes: applications of the principles of nucleic acid hybridization.DNA探针：核酸杂交原理的应用
Crit Rev Biochem Mol Biol. 1991;26(3-4):227-59. doi: 10.3109/10409239109114069.
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