• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

分子信标：一种基于荧光的溶液杂交技术用于瘤胃细菌生态学研究的试验

Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.

作者信息

Schofield P, Pell A N, Krause D O

机构信息

Department of Animal Science, Cornell University, Ithaca, New York 14853, USA.

出版信息

Appl Environ Microbiol. 1997 Mar;63(3):1143-7. doi: 10.1128/aem.63.3.1143-1147.1997.

DOI:10.1128/aem.63.3.1143-1147.1997
PMID:9055429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC168404/
Abstract

Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.

摘要

分子信标是为溶液而非膜杂交开发的荧光探针。我们研究了这些探针在瘤胃微生物生态学研究中的实用性。测试了两种纤维素分解菌，即白色瘤胃球菌和琥珀酸纤维杆菌。在白色瘤胃球菌8和琥珀酸纤维杆菌S85共培养物的竞争实验中，膜杂交和溶液杂交给出了相似的结果。

相似文献

1
Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.分子信标：一种基于荧光的溶液杂交技术用于瘤胃细菌生态学研究的试验
Appl Environ Microbiol. 1997 Mar;63(3):1143-7. doi: 10.1128/aem.63.3.1143-1147.1997.
2
Competitions between Fibrobacter succinogenes, Ruminococcus flavefaciens, and Ruminoccus albus in a Gnotobiotic Sheep Model Revealed by Multi-Omic Analyses.通过多组学分析揭示共生绵羊模型中纤维丁酸弧菌、黄色瘤胃球菌和白色瘤胃球菌之间的竞争。
mBio. 2021 Mar 3;12(2):e03533-20. doi: 10.1128/mBio.03533-20.
3
The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production.利用靶向16S rRNA的寡核苷酸探针研究瘤胃纤维分解菌之间的竞争：瘤胃球菌属探针的开发及细菌素产生的证据
Appl Environ Microbiol. 1994 Oct;60(10):3688-96. doi: 10.1128/aem.60.10.3688-3696.1994.
4
Competition for cellulose among three predominant ruminal cellulolytic bacteria under substrate-excess and substrate-limited conditions.在底物过量和底物受限条件下三种主要瘤胃纤维素分解菌对纤维素的竞争
Appl Environ Microbiol. 1997 Feb;63(2):734-42. doi: 10.1128/aem.63.2.734-742.1997.
5
Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens.瘤胃纤维素分解菌的竞争性聚合酶链反应检测方法的开发与应用：琥珀酸丝状杆菌、白色瘤胃球菌和黄色瘤胃球菌
FEMS Microbiol Lett. 2001 Nov 13;204(2):361-6. doi: 10.1111/j.1574-6968.2001.tb10911.x.
6
The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: pure-culture studies with cellulose and alkaline peroxide-treated wheat straw.使用靶向16S rRNA的寡核苷酸探针研究瘤胃纤维分解菌之间的竞争：纤维素和碱性过氧化氢处理麦秸的纯培养研究
Appl Environ Microbiol. 1994 Oct;60(10):3697-703. doi: 10.1128/aem.60.10.3697-3703.1994.
7
Competition among three predominant ruminal cellulolytic bacteria in the absence or presence of non-cellulolytic bacteria.在有无非纤维素分解菌的情况下，三种主要瘤胃纤维素分解菌之间的竞争。
Microbiology (Reading). 2001 Jan;147(Pt 1):21-30. doi: 10.1099/00221287-147-1-21.
8
Competition for cellobiose among three predominant ruminal cellulolytic bacteria under substrate-excess and substrate-limited conditions.在底物过量和底物受限条件下三种主要瘤胃纤维素分解菌对纤维二糖的竞争
Appl Environ Microbiol. 1997 Feb;63(2):743-8. doi: 10.1128/aem.63.2.743-748.1997.
9
16S rDNA sequencing of Ruminococcus albus and Ruminococcus flavefaciens: design of a signature probe and its application in adult sheep.白色瘤胃球菌和黄色瘤胃球菌的16S rDNA测序：一种特异性探针的设计及其在成年绵羊中的应用
Microbiology (Reading). 1999 Jul;145 ( Pt 7):1797-1807. doi: 10.1099/13500872-145-7-1797.
10
Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria.在与非纤维分解性瘤胃细菌共培养条件下对琥珀酸纤维杆菌S85中基因表达的监测。
Arch Microbiol. 2015 Mar;197(2):269-76. doi: 10.1007/s00203-014-1049-0. Epub 2014 Oct 30.

引用本文的文献

1
Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus.基于分子信标的实时等温扩增（MBRTIA）技术在同时检测蜡样芽孢杆菌和金黄色葡萄球菌中的应用。
J Food Sci Technol. 2015 Jul;52(7):4642-6. doi: 10.1007/s13197-014-1525-1. Epub 2014 Aug 20.
2
Detection and quantification of gene expression in environmental bacteriology.环境细菌学中基因表达的检测与定量分析
Appl Environ Microbiol. 2004 Jul;70(7):3795-806. doi: 10.1128/AEM.70.7.3795-3806.2004.
3
Use of DNA and peptide nucleic acid molecular beacons for detection and quantification of rRNA in solution and in whole cells.利用DNA和肽核酸分子信标检测和定量溶液及全细胞中的rRNA。
Appl Environ Microbiol. 2003 Sep;69(9):5673-8. doi: 10.1128/AEM.69.9.5673-5678.2003.
4
Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples.实时定量聚合酶链反应结合分子信标与巢式聚合酶链反应及培养方法检测牛粪便样本中副结核分枝杆菌的比较
J Clin Microbiol. 2002 Jan;40(1):287-91. doi: 10.1128/JCM.40.1.287-291.2002.

本文引用的文献

1
Ruminococcus flavefaciens Cell Coat and Adhesion to Cotton Cellulose and to Cell Walls in Leaves of Perennial Ryegrass (Lolium perenne).黄色瘤胃球菌的细胞外壳及其对棉花纤维素和多年生黑麦草（Lolium perenne）叶片细胞壁的粘附性。
Appl Environ Microbiol. 1978 Jan;35(1):156-65. doi: 10.1128/aem.35.1.156-165.1978.
2
The anaerobic mesophilic cellulolytic bacteria.厌氧嗜温性纤维素分解菌。
Bacteriol Rev. 1950 Mar;14(1):1-49. doi: 10.1128/br.14.1.1-49.1950.
3
Molecular beacons: probes that fluoresce upon hybridization.分子信标：杂交时发出荧光的探针。
Nat Biotechnol. 1996 Mar;14(3):303-8. doi: 10.1038/nbt0396-303.
4
An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.一种用于评估专性氨基酸发酵细菌在瘤胃氨基酸脱氨作用中角色的rRNA方法。
Appl Environ Microbiol. 1996 Mar;62(3):815-21. doi: 10.1128/aem.62.3.815-821.1996.
5
Utilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria.三种主要瘤胃纤维素分解菌对单个纤维糊精的利用情况
Appl Environ Microbiol. 1996 Mar;62(3):1084-8. doi: 10.1128/aem.62.3.1084-1088.1996.
6
Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations.针对纤维素分解菌属纤维杆菌的分类群特异性探针揭示了丰富且新颖的与马相关的菌群。
Appl Environ Microbiol. 1995 Apr;61(4):1348-51. doi: 10.1128/aem.61.4.1348-1351.1995.
7
The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production.利用靶向16S rRNA的寡核苷酸探针研究瘤胃纤维分解菌之间的竞争：瘤胃球菌属探针的开发及细菌素产生的证据
Appl Environ Microbiol. 1994 Oct;60(10):3688-96. doi: 10.1128/aem.60.10.3688-3696.1994.
8
Commentary on the Hungate technique for culture of anaerobic bacteria.关于厌氧细菌培养的亨盖特技术的述评
Am J Clin Nutr. 1972 Dec;25(12):1324-8. doi: 10.1093/ajcn/25.12.1324.
9
Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.基于系统发育的杂交探针在瘤胃微生物生态学研究中的应用。
Appl Environ Microbiol. 1988 May;54(5):1079-84. doi: 10.1128/aem.54.5.1079-1084.1988.
10
DNA probes: applications of the principles of nucleic acid hybridization.DNA探针：核酸杂交原理的应用
Crit Rev Biochem Mol Biol. 1991;26(3-4):227-59. doi: 10.3109/10409239109114069.