评估具有胆汁酸7α-脱羟基活性的粪便细菌中是否存在bai样基因。
Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes.
作者信息
Doerner K C, Takamine F, LaVoie C P, Mallonee D H, Hylemon P B
机构信息
Department of Microbiology and Immunology, Virginia Commonwealth University-Medical College of Virginia, Richmond 23298-0678, USA.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):1185-8. doi: 10.1128/aem.63.3.1185-1188.1997.
Abstract
Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 12708.
摘要
真杆菌属菌株VPI 12708有几个胆汁酸诱导型（bai）基因，这些基因编码胆汁酸7α-脱羟基化（7α DeOH）途径中的酶。对12株7α DeOH阳性肠道细菌菌株进行了7α DeOH活性检测，并对13株菌株进行了与bai基因杂交的测试。这些菌株中胆酸7α DeOH活性差异很大（>100倍）。Southern杂交实验表明，从13株菌株中的7株制备的DNA与真杆菌属菌株VPI 12708的至少一个bai基因杂交。
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