使用一种新型的鲎蛋白（T-GBP）进行血浆（1→3）-β-D-葡聚糖检测以及对真菌细胞壁中（1→3）-β-D-葡聚糖进行免疫组织化学染色，该鲎蛋白能特异性结合（1→3）-β-D-葡聚糖。
Plasma (1-->3)-beta-D-glucan assay and immunohistochemical staining of (1-->3)-beta-D-glucan in the fungal cell walls using a novel horseshoe crab protein (T-GBP) that specifically binds to (1-->3)-beta-D-glucan.
作者信息
Tamura H, Tanaka S, Ikeda T, Obayashi T, Hashimoto Y
机构信息
Tokyo Research Institute, Seikagaku Corporation, Japan.
出版信息
J Clin Lab Anal. 1997;11(2):104-9. doi: 10.1002/(SICI)1098-2825(1997)11:2<104::AID-JCLA6>3.0.CO;2-B.
Abstract
A highly sensitive enzyme-linked immunosorbent assay specific to (1-->3)-beta-D-glucans (GBP-ELISA) has been developed using a novel (1-->3)-beta-D-glucan-binding protein (T-GBP), which was purified from the amebocyte lysate of the Japanese horseshoe crab, Tachypleus tridentatus. This method allowed quantitation of the glucans in a concentration range of 0.1-1,000 ng/ml, regardless of linear and branched structures, and was applied to determine the amounts of (1-->3)-beta-D-glucan in human and animal plasmas for diagnosis of fungemia. High levels of plasma glucan contents in clinical samples were found to be correlated closely with the severity of fungal infection. T-GBP was successfully utilized for indirect immunofluorescence staining of (1-->3)-beta-D-glucan in Candida albicans cell walls.
摘要
利用从日本鲎（Tachypleus tridentatus）的血细胞裂解物中纯化得到的新型（1→3）-β-D-葡聚糖结合蛋白（T-GBP），开发了一种对（1→3）-β-D-葡聚糖具有高度特异性的酶联免疫吸附测定法（GBP-ELISA）。该方法能够对浓度范围为0.1-1000 ng/ml的葡聚糖进行定量，而不论其线性和分支结构如何，并被应用于测定人和动物血浆中（1→3）-β-D-葡聚糖的含量，以诊断真菌血症。临床样本中血浆葡聚糖含量的高水平被发现与真菌感染的严重程度密切相关。T-GBP成功用于白色念珠菌细胞壁中（1→3）-β-D-葡聚糖的间接免疫荧光染色。
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Plasma (1-->3)-beta-D-glucan assay and immunohistochemical staining of (1-->3)-beta-D-glucan in the fungal cell walls using a novel horseshoe crab protein (T-GBP) that specifically binds to (1-->3)-beta-D-glucan.使用一种新型的鲎蛋白（T-GBP）进行血浆（1→3）-β-D-葡聚糖检测以及对真菌细胞壁中（1→3）-β-D-葡聚糖进行免疫组织化学染色，该鲎蛋白能特异性结合（1→3）-β-D-葡聚糖。
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