短短芽孢杆菌在模拟微重力条件下产生短杆菌肽S

Gramicidin S production by Bacillus brevis in simulated microgravity.

作者信息

Fang A, Pierson D L, Mishra S K, Koenig D W, Demain A L

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

出版信息

Curr Microbiol. 1997 Apr;34(4):199-204. doi: 10.1007/s002849900168.

DOI:10.1007/s002849900168

PMID:9058537

Abstract

In a continuing study of microbial secondary metabolism in simulated microgravity, we have examined gramicidin S (GS) production by Bacillus brevis strain Nagano in NASA High Aspect Rotating Vessels (HARVs), which are designed to simulate some aspects of microgravity. Growth and GS production were found to occur under simulated microgravity. When performance under simulated microgravity was compared with that under normal gravity conditions in the bioreactors, GS production was found to be unaffected by simulated microgravity. The repressive effect of glycerol in flask fermentations was not observed in the HARV. Thus the negative effect of glycerol on specific GS formation is dependent on shear and/or vessel geometry, not gravity.

摘要

在一项关于模拟微重力下微生物次级代谢的持续研究中，我们检测了短小芽孢杆菌长野菌株在NASA高纵横比旋转容器（HARV）中短杆菌肽S（GS）的产生情况，该容器旨在模拟微重力的某些方面。发现在模拟微重力条件下会发生生长和GS的产生。当将模拟微重力下的性能与生物反应器中正常重力条件下的性能进行比较时，发现GS的产生不受模拟微重力的影响。在HARV中未观察到甘油在摇瓶发酵中的抑制作用。因此，甘油对特定GS形成的负面影响取决于剪切力和/或容器几何形状，而非重力。