Nicola A V, Peng C, Lou H, Cohen G H, Eisenberg R J
Department of Microbiology, University of Pennsylvania, Philadelphia 19104, USA.
J Virol. 1997 Apr;71(4):2940-6. doi: 10.1128/JVI.71.4.2940-2946.1997.
Soluble forms of herpes simplex virus (HSV) glycoprotein D (gD) block viral penetration. Likewise, most HSV strains are sensitive to gD-mediated interference by cells expressing gD. The mechanism of both forms of gD-mediated inhibition is thought to be at the receptor level. We analyzed the ability of different forms of soluble, truncated gD (gDt) to inhibit infection by different strains of HSV-1 and HSV-2. Strains that were resistant to gD-mediated interference were also resistant to inhibition by gDt, thereby suggesting a link between these two phenomena. Virion gD was the major viral determinant for resistance to inhibition by gDt. An insertion-deletion mutant, gD-1(delta 290-299t), had an enhanced inhibitory activity against most strains tested. The structure and function of gDt proteins derived from the inhibition-resistant viruses rid1 and ANG were analyzed. gD-1(ridlt) and gD-1(ANGt) had a potent inhibitory effect on plaque formation by wild-type strains of HSV but, surprisingly, little or no effect on their parental strains. As measured by quantitative enzyme-linked immunosorbent assay with a diverse panel of monoclonal antibodies, the antigenic structures of gD-1(rid1t) and gD-1(ANGt) were divergent from that of the wild type yet were similar to each other and to that of gD-1 (delta 290-299t). Thus, three different forms of gD have common antigenic changes that correlate with enhanced inhibitory activity against HSV. We conclude that inhibition of HSV infectivity by soluble gD is influenced by the antigenic conformation of the blocking gDt as well as the form of gD in the target virus.
单纯疱疹病毒（HSV）糖蛋白D（gD）的可溶性形式可阻断病毒穿透。同样，大多数HSV毒株对表达gD的细胞介导的gD干扰敏感。这两种形式的gD介导的抑制机制被认为是在受体水平。我们分析了不同形式的可溶性截短gD（gDt）抑制不同株HSV-1和HSV-2感染的能力。对gD介导的干扰具有抗性的毒株对gDt的抑制也具有抗性，从而表明这两种现象之间存在联系。病毒粒子gD是对gDt抑制产生抗性的主要病毒决定因素。一个插入-缺失突变体gD-1（delta 290-299t）对大多数测试毒株具有增强的抑制活性。分析了来自抗抑制病毒rid1和ANG的gDt蛋白的结构和功能。gD-1（ridlt）和gD-1（ANGt）对HSV野生型毒株的噬斑形成有强大的抑制作用，但令人惊讶的是，对其亲本毒株几乎没有影响。通过用多种单克隆抗体进行定量酶联免疫吸附测定，gD-1（rid1t）和gD-1（ANGt）的抗原结构与野生型不同，但彼此相似且与gD-1（delta 290-299t）相似。因此，三种不同形式的gD具有共同的抗原变化，这些变化与对HSV的增强抑制活性相关。我们得出结论，可溶性gD对HSV感染性的抑制受阻断gDt的抗原构象以及靶病毒中gD形式的影响。
