鉴定由CELO腺病毒编码的一种新型抗凋亡蛋白GAM-1。
Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus.
作者信息
Chiocca S, Baker A, Cotten M
机构信息
Institute for Molecular Pathology, Vienna, Austria.
出版信息
J Virol. 1997 Apr;71(4):3168-77. doi: 10.1128/JVI.71.4.3168-3177.1997.
Abstract
We have developed a simple screening method to identify genes that mimic bcl-2 or adenovirus E1B 19K in enhancing cell survival after transfection and have used this method to identify such a gene in the avian adenovirus CELO. The gene encodes a novel 30-kDa nuclear protein, which we have named GAM-1, that functions comparably to Bcl-2 and adenovirus E1B 19K in blocking apoptosis. However, GAM-1 has no sequence homology to Bcl-2, E1B 19K, or any other known antiapoptotic proteins and thus defines a novel antiapoptotic function.
摘要
我们开发了一种简单的筛选方法，用于鉴定在转染后能模拟bcl-2或腺病毒E1B 19K增强细胞存活能力的基因，并利用该方法在禽腺病毒CELO中鉴定出了这样一个基因。该基因编码一种新的30 kDa核蛋白，我们将其命名为GAM-1，它在阻断细胞凋亡方面的功能与Bcl-2和腺病毒E1B 19K相当。然而，GAM-1与Bcl-2、E1B 19K或任何其他已知的抗凋亡蛋白没有序列同源性，因此定义了一种新的抗凋亡功能。
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