Zvaifler N J, Tsai V, Alsalameh S, von Kempis J, Firestein G S, Lotz M
Department of Medicine, University of California, San Diego, La Jolla 92093-0664, USA.
Am J Pathol. 1997 Mar;150(3):1125-38.
A distinctive cell was identified from sites of rheumatoid arthritis cartilage injury. Similar cells are not found in lesions of osteoarthritis cartilage. We have designated them as pannocytes (PCs). Their rhomboid morphology differs from the bipolar shape of fibroblast-like synoviocytes or the spherical configuration of primary human articular chondrocytes. Chondrocytes are short-lived, whereas the original PC line grew for 25 passages before becoming senescent. Features in common with cultured primary chondrocytes include maximal proliferation in response to transforming growth factor-beta a catabolic response to interleukin-1 beta, collagenase production, and mRNA for the induced lymphocyte antigen and inducible nitric oxide synthase. Despite the presence of the inducible nitric oxide synthase message, PCs do not produce NO either constitutively or when cytokine stimulated. Each of the mesenchymal cells, fibroblast-like synoviocytes, primary chondrocytes, and PCs have the gene for type I collagen, but the type II collagen gene is detected only in primary chondrocytes. PCs can be distinguished from fibroblast-like synoviocytes and primary chondrocytes by their morphology, bright VCAM-1 staining, and growth response to cytokines and growth factors. Their prolonged life span in vitro suggests that PCs might represent an earlier stage of mesenchymal cell differentiation, and they could have a heretofore unrecognized role in rheumatoid arthritis joint destruction.
在类风湿性关节炎软骨损伤部位鉴定出一种独特的细胞。骨关节炎软骨损伤部位未发现类似细胞。我们将它们命名为滑膜细胞（PCs）。它们的菱形形态不同于成纤维细胞样滑膜细胞的双极形状或原代人关节软骨细胞的球形结构。软骨细胞寿命短暂，而原始的PC细胞系在衰老前能传代25次。与培养的原代软骨细胞的共同特征包括对转化生长因子-β的最大增殖反应、对白细胞介素-1β的分解代谢反应、胶原酶产生以及诱导淋巴细胞抗原和诱导型一氧化氮合酶的信使核糖核酸。尽管存在诱导型一氧化氮合酶信息，但PCs无论是组成性还是细胞因子刺激时都不产生一氧化氮。间充质细胞、成纤维细胞样滑膜细胞、原代软骨细胞和PCs都有I型胶原基因，但II型胶原基因仅在原代软骨细胞中检测到。PCs可通过其形态、明亮的血管细胞黏附分子-1染色以及对细胞因子和生长因子的生长反应与成纤维细胞样滑膜细胞和原代软骨细胞区分开来。它们在体外的延长寿命表明PCs可能代表间充质细胞分化的早期阶段，并且它们在类风湿性关节炎关节破坏中可能具有迄今未被认识的作用。
