Waller K P, Colditz I G, Flapper P, Seow H F
Swedish University of Agricultural Sciences, Department of Obstetrics and Gynaecology, Uppsala, Sweden.
Vet Res Commun. 1997 Feb;21(2):101-15. doi: 10.1023/a:1005757115292.
The accumulation of leukocytes, ovine serum albumin and the cytokines interleukin-1 beta(IL-1 beta), tumour necrosis factor-alpha(TNF-alpha), interleukin-8 (IL-8), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon-gamma(IFN-gamma) was studied during endotoxin-induced inflammation in lactating and dry ovine udders, and in the teat cisterns of dry ewes after surgical closure of the passage between the teat and udder cisterns. Samples were taken before infusion and hourly up to 10 h after infusion of 0.1, 1 or 10 micrograms of endotoxin, or infusion of pyrogen-free saline (PFS) as a control. Rectal temperatures were measured. A significant dose- and time-dependent accumulation of leukocytes, mainly neutrophils, was observed in the lactating udders and in the teat cisterns. In the dry udders, the leukocyte accumulation was significant for time but not for dose. Peak numbers of cells were reached at 3-4 h in the dry udders and in the teat cisterns, but not until 10 h after infusion in the lactating udders. The changes in the ovine serum albumin concentrations mostly paralleled changes in leukocyte numbers. A role was indicated for TNF-alpha, IL-8 and GM-CSF, but not for IL-1 beta and IFN-gamma, during endotoxin-induced inflammation in the ovine udder. Release of TNF-alpha, IL-8 and GM-CSF was most prominent in lactating udders, peaking at 2 or 3 h after infusion, but was also detected in dry udders and teat cisterns. Detectable levels of IL-1 beta and IFN-gamma were occasionally found in all three groups.
在内毒素诱导的泌乳期和干奶期绵羊乳房炎症期间，以及在手术封闭乳头与乳房贮乳池之间通道后干奶期母羊的乳头管中，研究了白细胞、绵羊血清白蛋白以及细胞因子白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和干扰素-γ（IFN-γ）的积累情况。在输注0.1、1或10微克内毒素或输注无热原生理盐水（PFS）作为对照之前以及输注后每小时采集样本，直至输注后10小时。测量直肠温度。在泌乳期乳房和乳头管中观察到白细胞（主要是中性粒细胞）显著的剂量和时间依赖性积累。在干奶期乳房中，白细胞积累在时间上有显著差异，但在剂量上无显著差异。干奶期乳房和乳头管中的细胞数量在3 - 4小时达到峰值，但泌乳期乳房在输注后10小时才达到峰值。绵羊血清白蛋白浓度的变化大多与白细胞数量的变化平行。在内毒素诱导的绵羊乳房炎症期间，表明TNF-α、IL-8和GM-CSF发挥了作用，但IL-1β和IFN-γ未发挥作用。TNF-α、IL-8和GM-CSF的释放在泌乳期乳房中最为显著，在输注后2或3小时达到峰值，但在干奶期乳房和乳头管中也有检测到。在所有三组中偶尔都能检测到可检测水平的IL-1β和IFN-γ。
