日本脑炎病毒第二种基因型在澳大拉西亚地区的出现。

The appearance of a second genotype of Japanese encephalitis virus in the Australasian region.

作者信息

Pyke A T, Williams D T, Nisbet D J, van den Hurk A F, Taylor C T, Johansen C A, Macdonald J, Hall R A, Simmons R J, Mason R J, Lee J M, Ritchie S A, Smith G A, Mackenzie J S

机构信息

Public Health Virology, Queensland Health Scientific Services, Coopers Plains, Australia.

出版信息

Am J Trop Med Hyg. 2001 Dec;65(6):747-53. doi: 10.4269/ajtmh.2001.65.747.

DOI:10.4269/ajtmh.2001.65.747

PMID:11791969

Abstract

In mid-January 2000, the reappearance of Japanese encephalitis (JE) virus activity in the Australasian region was first demonstrated by the isolation of JE virus from 3 sentinel pigs on Badu Island in the Torres Strait. Further evidence of JE virus activity was revealed through the isolation of JE virus from Culex gelidus mosquitoes collected on Badu Island and the detection of specific JE virus neutralizing antibodies in 3 pigs from Saint Pauls community on Moa Island. Nucleotide sequencing and phylogenetic analyses of the premembrane and envelope genes were performed which showed that both the pig and mosquito JE virus isolates (TS00 and TS4152, respectively) clustered in genotype I, along with northern Thai, Cambodian, and Korean isolates. All previous Australasian JE virus isolates belong to genotype II, along with Malaysian and Indonesian isolates. Therefore, for the first time, the appearance and transmission of a second genotype of JE virus in the Australasian region has been demonstrated.

摘要

2000年1月中旬，澳大利亚地区日本脑炎（JE）病毒活动再次出现，这首先通过从托雷斯海峡巴杜岛的3头哨兵猪中分离出JE病毒得到证实。通过从巴杜岛采集的环纹库蚊中分离出JE病毒，以及在莫阿岛圣保罗社区的3头猪中检测到特异性JE病毒中和抗体，进一步揭示了JE病毒活动的证据。对前膜和包膜基因进行了核苷酸测序和系统发育分析，结果表明，猪和蚊子的JE病毒分离株（分别为TS00和TS4152）与泰国北部、柬埔寨和韩国的分离株一起，聚集在基因型I中。以前所有澳大利亚的JE病毒分离株都与马来西亚和印度尼西亚的分离株一起属于基因型II。因此，首次证明了澳大利亚地区第二种基因型JE病毒的出现和传播。