Bloom S L, Dias K M, Bawdon R E, Gilstrap L C
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9032, USA.
Am J Obstet Gynecol. 1997 Feb;176(2):291-3. doi: 10.1016/s0002-9378(97)70487-3.
Our purpose was to measure the transfer of lamivudine ([-]-2'-deoxy-3'-thiacytidine) across the human placenta both alone and in the presence of zidovudine.
Nine placentas from term, elective cesarean deliveries were analyzed with use of the ex vivo single cotyledon perfusion system. Antipyrine was used as the reference compound to measure the clearance index values of lamivudine alone and in combination with zidovudine. Lamivudine concentrations in the perfusates and tissues were quantified by high-pressure liquid chromatography.
The clearance index of lamivudine at a maternal concentration of 1.39 micrograms/ml was 0.23 +/- 0.14. At a peak concentration of 14.68 micrograms/ml the clearance index was 0.14 +/- 0.06. These index values did not significantly change in the presence of 1 or 10 micrograms/ml of zidovudine. In a closed recirculating system the fetal lamivudine concentration increased as more lamivudine was added to the maternal perfusate. The addition of zidovudine did not influence this transfer.
Lamivudine appears to cross the placenta by simple diffusion and its transfer does not appear to be altered by zidovudine.
我们的目的是测量拉米夫定（[-]-2'-脱氧-3'-硫代胞苷）单独以及在齐多夫定存在的情况下通过人胎盘的转运情况。
使用离体单叶胎盘灌注系统分析了9例足月择期剖宫产分娩的胎盘。安替比林用作参考化合物，以测量拉米夫定单独以及与齐多夫定联合使用时的清除指数值。通过高压液相色谱法定量测定灌注液和组织中的拉米夫定浓度。
母体浓度为1.39微克/毫升时，拉米夫定的清除指数为0.23±0.14。在峰值浓度为14.68微克/毫升时，清除指数为0.14±0.06。在存在1或10微克/毫升齐多夫定的情况下，这些指数值没有显著变化。在封闭的循环系统中，随着向母体灌注液中添加更多的拉米夫定，胎儿拉米夫定浓度增加。添加齐多夫定不影响这种转运。
拉米夫定似乎通过简单扩散穿过胎盘，其转运似乎不受齐多夫定的影响。
