Kasik J W
Department of Pediatrics, Metro Health Medical Center, Case Western Reserve University, Room R-249, 2500 Metro Health Drive, Cleveland, 44109 OH, USA.
Biochem J. 1998 Mar 1;330 ( Pt 2)(Pt 2):947-50. doi: 10.1042/bj3300947.
A cDNA was cloned from a pregnant mouse uterus cDNA library. On conceptual translation, the cDNA has one long open reading frame that predicts a novel protein of 606 amino acids. This protein is principally composed of two CUB domains and a ZP domain; motifs found in proteins implicated in egg-sperm recognition. Probes derived from the cDNA were used to conduct Northern hybridizations. The expression of this mRNA is temporal; message first appears in the uterus 6 days prior to birth, it increases each subsequent day to attain maximal levels at 3 days prior to birth and then abruptly decreases during the last 3 days of pregnancy. The expression of this mRNA is restricted; message is abundant in the uterus during late pregnancy, but it is not found in non-pregnant uterus or in a variety of adult or fetal tissues. The temporo-spatial expression of this pregnant uterus specific mRNA and the consolidation in the predicted protein of two motifs implicated in early pregnancy events suggests that the product of the gene represented by this mRNA may play an important role in events that transpire during late pregnancy.
从怀孕小鼠子宫cDNA文库中克隆出一个cDNA。经概念性翻译，该cDNA有一个长的开放阅读框，预测编码一个含606个氨基酸的新蛋白质。此蛋白质主要由两个CUB结构域和一个ZP结构域组成；这些基序存在于与精卵识别相关的蛋白质中。从该cDNA衍生的探针用于进行Northern杂交。这种mRNA的表达具有时间性；其信息最早在出生前6天出现在子宫中，随后每天增加，在出生前3天达到最高水平，然后在怀孕的最后3天突然下降。这种mRNA的表达具有局限性；在怀孕后期子宫中该信息丰富，但在未怀孕的子宫或多种成年或胎儿组织中未发现。这种怀孕子宫特异性mRNA的时空表达以及预测蛋白质中与早期妊娠事件相关的两个基序的整合表明，该mRNA所代表基因的产物可能在怀孕后期发生的事件中起重要作用。
