Gosalbes M J, Monedero V, Alpert C A, Pérez-Martínez G
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Polígono de la Coma sln, Burjasot, Valencia, Spain.
FEMS Microbiol Lett. 1997 Mar 1;148(1):83-9. doi: 10.1111/j.1574-6968.1997.tb10271.x.
The chromosomally encoded lactose-specific phosphoenol pyruvate-dependent phosphotransferase system (PTS) has been investigated in Lactobacillus casei ATCC 393 [pLZ15-] and it was considered an excellent system to study the regulation of the lactose operon. This chromosomal operon has been cloned and sequenced, being 99% homologous to that encoded on the plasmid pLZ64. Expression of the lactose operon in different mutants of L. casei ATCC 393 [pLZ15-] and primer extension analysis revealed that it is subject to a dual regulation: (i) glucose repression possibly mediated by CcpA and PTS elements, and (ii) induction by lactose through transcriptional antitermination.
已对干酪乳杆菌ATCC 393 [pLZ15-]中染色体编码的乳糖特异性磷酸烯醇丙酮酸依赖性磷酸转移酶系统（PTS）进行了研究，并且它被认为是研究乳糖操纵子调控的优良系统。该染色体操纵子已被克隆和测序，与质粒pLZ64上编码的操纵子具有99%的同源性。干酪乳杆菌ATCC 393 [pLZ15-]不同突变体中乳糖操纵子的表达以及引物延伸分析表明，它受到双重调控：（i）可能由CcpA和PTS元件介导的葡萄糖阻遏，以及（ii）通过转录抗终止作用由乳糖诱导。
