Tsukada Y, Hibi N, Sakashita S, Abe K, Hirai H
Int J Cancer. 1979 Sep 15;24(3):341-8. doi: 10.1002/ijc.2910240312.
Five rat yolk sac tumor cell lines were cloned from a yolk sac tumor line which originally arose following fetectomy. The doubling time of each of the cloned tumor lines was about 50 h. All of the cloned tumor cell lines synthesized and secreted AFP and albumin but there was a gradual decrease in the synthesis of these proteins during serial passage. The cells formed clusters which looked like vitelline ducts or the parietal yolk sac and they also had basement membranes which closely resembled Reichert's membrane. When the cloned cell lines were cultivated in the presence of 1 mM dibutyryl cyclic-AMP, myotube-like and neuron-like cells appeared. Acetylcholine esterase and creatine phosphokinase activity were present when myotube-like cells were present whereas acetylcholine esterase activity predominated when neuron-like cells were present.
从最初因胚胎切除后出现的卵黄囊瘤细胞系中克隆出了5个大鼠卵黄囊瘤细胞系。每个克隆的肿瘤细胞系的倍增时间约为50小时。所有克隆的肿瘤细胞系均合成并分泌甲胎蛋白（AFP）和白蛋白，但在连续传代过程中这些蛋白质的合成逐渐减少。这些细胞形成簇状，看起来像卵黄管或壁层卵黄囊，并且它们还具有与赖歇特氏膜非常相似的基底膜。当克隆的细胞系在1 mM二丁酰环磷腺苷（dibutyryl cyclic - AMP）存在的情况下培养时，会出现肌管样细胞和神经元样细胞。当存在肌管样细胞时，存在乙酰胆碱酯酶和肌酸磷酸激酶活性，而当存在神经元样细胞时，乙酰胆碱酯酶活性占主导。
