Sakagami H, Satoh K
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1997 Jan-Feb;17(1A):221-4.
The addition of two antioxidants, ascorbic acid or gallic acid to distilled water resulted in the rapid increase in the reduction potential, which a few seconds later was replaced with much higher oxidation potential. The addition of these compounds to culture medium dose-dependently increased the oxidation potential only. Ascorbic acid analogs, which had apoptosis-inducing activity, showed similar oxidation potential, whereas inactive analogs did not. The combination of ascorbate and hydrogen peroxide additively enhanced both the oxidation potential and cytotoxic activity. These data demonstrate that both ascorbic acid and gallic acid act as prooxidants for the induction of apoptotic cell death.
在蒸馏水中添加两种抗氧化剂，即抗坏血酸或没食子酸，会导致还原电位迅速升高，几秒钟后又被更高的氧化电位所取代。将这些化合物添加到培养基中仅会剂量依赖性地增加氧化电位。具有诱导凋亡活性的抗坏血酸类似物显示出相似的氧化电位，而无活性的类似物则没有。抗坏血酸盐和过氧化氢的组合可相加增强氧化电位和细胞毒性活性。这些数据表明，抗坏血酸和没食子酸均作为促氧化剂诱导凋亡性细胞死亡。
