Heyerdahl H, Wang I, Liu D L, Berg R, Andersson-Engels S, Peng Q, Moan J, Svanberg S, Svanberg K
Department of Surgical Oncology, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Cancer Lett. 1997 Jan 30;112(2):225-31. doi: 10.1016/s0304-3835(96)04576-4.
Laser-induced fluorescence (LIF) for in vivo point monitoring and fluorescence microscopy incorporating a CCD camera were used to study the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in tumours. Fluorescence in a chemically-induced adenocarcinoma in the liver of rats and in an aggressive basal cell carcinoma in a patient were studied after intravenous injection of ALA at a dose of 30 mg/kg body weight. The LIF technique demonstrated slightly more ALA-induced PpIX fluorescence in the tumour than in the surrounding normal liver and abdominal muscle of rats. The visible parts of the human basal cell carcinoma exhibited strong ALA-induced fluorescence, while this fluorescence was much weaker in the necrotic areas of the tumour and in the surrounding normal skin.
利用激光诱导荧光（LIF）进行体内点监测，并结合电荷耦合器件（CCD）相机的荧光显微镜来研究5-氨基酮戊酸（ALA）诱导的原卟啉IX（PpIX）在肿瘤中的荧光分布。在以30mg/kg体重的剂量静脉注射ALA后，对大鼠肝脏化学诱导的腺癌以及一名患者侵袭性基底细胞癌中的荧光进行了研究。LIF技术显示，肿瘤中ALA诱导的PpIX荧光略多于大鼠周围正常肝脏和腹部肌肉中的荧光。人基底细胞癌的可见部分呈现出强烈的ALA诱导荧光，而在肿瘤坏死区域和周围正常皮肤中，这种荧光则弱得多。
