Riario-Sforza G G, Della Torre F, Antonicelli L, Bonifazi F, Giordano T, D'Amato G, Liccardi G, Bettini P, Incorvaia C
Allergy Unit, Buzzi Hospital, Milan, Italy.
Allergy Asthma Proc. 1997 Jan-Feb;18(1):23-8. doi: 10.2500/108854197778612790.
The prevalence of sensitization to cockroach (CR) was evaluated by skin prick test (SPT) in 1299 patients consecutively referred for rhinitis and asthma to five allergy centers in northern, central, and southern Italy. In patients with positive SPT to CR, an SPT with D. pteronyssinus extract was made. RAST inhibition was performed with pooled sera of subjects with positive SPT and RAST of at least Class 3 for CR and D. Pteronyssinus, to assess the cross-reactivity between these two allergenic sources. The protein content of allergen extracts was assayed by isoelectric focusing (IEF). A total of 170 patients (13%) had a positive SPT with CR extract, and 133 of them (78.2%) were also positive for D. pteronyssinus. RAST inhibition showed a cross-reactivity between CR and D. pteronyssinus, and IEF detected in the extracts employed a series of bands focusing at the same pI of CR and D. pteronyssinus relevant allergens. Therefore, the prevalence of sensitization to CR in the population studied, corresponding to 13%, suggests that CR extract might be added to standard SPT panels for respiratory allergy. However, the cross-reactivity with D. pteronyssinus calls for careful evaluation of the clinical importance of this sensitization.
在意大利北部、中部和南部的五个过敏中心，对1299例因鼻炎和哮喘连续转诊的患者进行了皮肤点刺试验（SPT），以评估对蟑螂（CR）的致敏率。对CR皮肤点刺试验呈阳性的患者，用屋尘螨提取物进行皮肤点刺试验。对皮肤点刺试验阳性且蟑螂和屋尘螨RAST至少为3级的受试者的混合血清进行RAST抑制试验，以评估这两种过敏原来源之间的交叉反应性。通过等电聚焦（IEF）测定过敏原提取物的蛋白质含量。共有170例患者（13%）蟑螂提取物皮肤点刺试验呈阳性，其中133例（78.2%）屋尘螨也呈阳性。RAST抑制试验显示蟑螂和屋尘螨之间存在交叉反应性，IEF在所用提取物中检测到一系列聚焦于与蟑螂和屋尘螨相关过敏原相同等电点的条带。因此，在所研究人群中对蟑螂的致敏率为13%，这表明蟑螂提取物可能应添加到呼吸道过敏的标准皮肤点刺试验组中。然而，与屋尘螨的交叉反应性需要仔细评估这种致敏的临床重要性。
