用配对发射体探测器二极管进行血红蛋白测定。

University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.

Anal Bioanal Chem. 2011 Mar;399(9):3293-7. doi: 10.1007/s00216-010-4358-4. Epub 2010 Nov 3.

Two ordinary green light-emitting diodes used as light emitter and detector coupled with simple voltmeter form a complete, cost-effective prototype of a photometric hemoglobinometer. The device has been optimized for cuvette assays of total hemoglobin (Hb) in diluted blood using three different chemical methods recommended for the needs of clinical analysis (namely Drabkin, lauryl sulfate, and dithionite methods). The utility of developed device for real analytics has been validated by the assays of total Hb content in human blood. The results of analysis are fully compatible with those obtained using clinically recommended method and clinical analyzer.

两个普通的绿色发光二极管，分别用作光源和探测器，再加上一个简单的电压表，就组成了一个完整的、具有成本效益的光电血红蛋白计原型。该设备针对使用三种不同的化学方法（即 Drabkin 法、十二烷基硫酸钠法和连二亚硫酸钠法）对稀释血液中的总血红蛋白（Hb）进行比色皿检测进行了优化，这些方法是为临床分析的需要而推荐的。所开发设备的实际分析实用性已通过对人血中总 Hb 含量的检测得到验证。分析结果与临床推荐方法和临床分析仪的结果完全一致。