Rosalen P L, Pearson S K, Bowen W H
Department of Dental Research, Rochester Caries Research Center, University of Rochester, NY 14642-8611, USA.
Arch Oral Biol. 1996 Nov;41(11):1003-10. doi: 10.1016/s0003-9969(96)00082-9.
The purpose was to explore the effects of combinations of copper, iron and fluoride (Cu, Fe and F) incorporated in sucrose by co-crystallization on caries development in the deslivated rat model and to examine acid formation by bacteria in the rat mouth. Ninety-six Sprague-Dawley rats were infected with Streptococcus sobrinus 6715 and desalivated when aged 26 days. Eight groups were placed in a König-Höfer programmed feeder and received 17 meals daily at hourly intervals, and essential nutrition (NCP No. 2) by gavage twice daily for 21 days. The groups received (1) plain sucrose, (2) F (8 parts/10(6)) co-crystallized with sucrose, (3) Fe (88 parts/10(6)) sucrose, (4) Cu (75 parts/10(6)) sucrose, (5) Cu + F sucrose, (6) Cu + L Fe sucrose, (7) F + Fe sucrose, and (8) Cu + Fe + F sucrose. At death the jaws were removed and sonicated in 0.9% saline solution for microbial assessment. In addition, organic acid assays were performed for each animal. Keyes smooth-surface and sulcal caries scores were lowest in the Cu + Fe + F sucrose group, but not statistically significantly different from those of the other Cu groups. The numbers of Strep. sobrinus found in the groups that received Cu, Cu + Fe, Cu + F, F + Fe and Cu + Fe + F sugar were lower than in the control group. Lactic acid was found in lower concentrations in Fe, Cu, Cu + F, Cu + Fe and F + Fe groups than in the other groups. It appears that combinations of Cu; Fe and F co-crystallized with sugar may have an additive effect in reducing the cariogenic potential of sugar by affecting lactic acid formation and reducing bacterial colonization.
目的是探讨通过共结晶将铜、铁和氟（Cu、Fe和F）掺入蔗糖中对去唾液大鼠模型龋齿发展的影响，并检测大鼠口腔中细菌的酸生成情况。96只Sprague-Dawley大鼠感染远缘链球菌6715，并在26日龄时进行去唾液处理。将8组大鼠放入König-Höfer程序化饲养器中，每隔1小时喂食17次，每天通过灌胃给予两次必需营养物（NCP No.2），持续21天。这些组分别接受（1）普通蔗糖，（2）与蔗糖共结晶的F（8份/10⁶），（3）Fe（88份/10⁶）蔗糖，（4）Cu（75份/10⁶）蔗糖，（5）Cu + F蔗糖，（6）Cu + 低剂量Fe蔗糖，（7）F + Fe蔗糖，以及（8）Cu + Fe + F蔗糖。处死大鼠后取出颌骨，在0.9%盐溶液中超声处理以进行微生物评估。此外，对每只动物进行有机酸测定。在Cu + Fe + F蔗糖组中，凯斯光滑面和沟裂龋评分最低，但与其他含铜组相比无统计学显著差异。在接受Cu、Cu + Fe、Cu + F、F + Fe和Cu + Fe + F糖的组中发现的远缘链球菌数量低于对照组。在Fe、Cu、Cu + F、Cu + Fe和F + Fe组中发现的乳酸浓度低于其他组。似乎与糖共结晶的Cu、Fe和F组合可能通过影响乳酸形成和减少细菌定植，在降低糖的致龋潜力方面具有累加效应。
