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用Etest法对葡萄球菌进行甲氧西林敏感性检测,并与琼脂稀释法及mecA检测法作比较。

Methicillin susceptibility testing of staphylococci by Etest and comparison with agar dilution and mecA detection.

作者信息

Weller T M, Crook D W, Crow M R, Ibrahim W, Pennington T H, Selkon J B

机构信息

Department of Medical Microbiology, Aberdeen Royal Infirmary, Foresterhill, UK.

出版信息

J Antimicrob Chemother. 1997 Feb;39(2):251-3. doi: 10.1093/jac/39.2.251.

Abstract

The susceptibility to methicillin of 44 Staphylococcus aureus and 120 coagulase-negative staphylococci (CNS) was determined by Etest, agar dilution and presence of the mecA gene. There was agreement between the results of all methods when testing S. aureus. However, discrepancies occurred with CNS when cultural methods were compared with presence of the mecA gene. mecA-positive isolates tested as resistant more often with agar dilution on Columbia agar plus 5% NaCl than by Etest.

摘要

采用Etest法、琼脂稀释法并检测mecA基因,测定了44株金黄色葡萄球菌和120株凝固酶阴性葡萄球菌(CNS)对甲氧西林的敏感性。检测金黄色葡萄球菌时,所有方法的结果一致。然而,在比较CNS的培养方法和mecA基因存在情况时出现了差异。与Etest法相比,mecA基因阳性分离株在含5%氯化钠的哥伦比亚琼脂上采用琼脂稀释法检测时更常表现为耐药。

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