Carbone A, Piantelli M, Musiani P, Pozzuoli R, Lauriola L
J Immunol Methods. 1979;29(3):245-51. doi: 10.1016/0022-1759(79)90312-0.
This paper is concerned with technical standardization in detecting Fc-IgM receptors on human T lymphocytes. We have investigated a number of factors of critical importance in obtaining easily reproducible and reliable estimates of the numbers of TM cells among human peripheral T lymphocytes. A point of major importance is optimal coating of erythrocytes by IgM molecules. For this condition to be met, particular attention is required when erythrocytes from animals other than the one used for obtaining antiserum are used to prepare EA-IgM. Determination of the agglutinating titer of IgM preparation is useful in determing optimal sensitizing dilutions. Full expression of Fc receptors is favoured when human cord serum is added to the medium. The influence of incubation period of EA-lymphocytes mixtures on TM counts has also been investigated.
本文关注人T淋巴细胞上Fc-IgM受体检测中的技术标准化问题。我们研究了许多关键因素，这些因素对于获得人外周T淋巴细胞中TM细胞数量的易于重复且可靠的估计至关重要。一个重要的点是IgM分子对红细胞的最佳包被。为满足这一条件，当使用除用于获得抗血清的动物之外的其他动物的红细胞来制备EA-IgM时，需要特别注意。IgM制剂凝集效价的测定有助于确定最佳致敏稀释度。当向培养基中加入人脐血清时，有利于Fc受体的充分表达。我们还研究了EA-淋巴细胞混合物孵育时间对TM计数的影响。
