Ponec M, Hasper I, Vianden G D, Bachra B N
Arch Dermatol Res (1975). 1977 Aug 22;259(2):125-34. doi: 10.1007/BF00557952.
Confluent cultures of normal primary human skin fibroblasts were incubated with various glucocorticosteroids which are in current use clinically for the treatment of various skin disorders. For all steroids concentrations were found at which collagen hydroxyproline formation was inhibited, while total protein synthesis was little affected. The concentration effective for inhibition was highest for hydrocortisone and lowest for clobetasol-17-propionate. All other steroids (hydrocortisone-17-butyrate, triamcinolone acetonide and betamethasone-17-valerate) showed medium effectiveness. Fluorination as such was not a factor in the degree of inhibition. The inhibition observed was shown to be independent of concomitant specific effects on cell proliferation or cell turnover. The possible implications of these findings on the therapeutic effects in psoriasis and the frequently occurring atrophic side-effects of these steroids are discussed.
将正常原代人皮肤成纤维细胞的融合培养物与目前临床上用于治疗各种皮肤疾病的各种糖皮质激素一起孵育。对于所有类固醇，都发现了抑制胶原蛋白羟脯氨酸形成的浓度，而总蛋白质合成受影响较小。抑制作用的有效浓度以氢化可的松最高，丙酸氯倍他索最低。所有其他类固醇（丁酸氢化可的松、曲安奈德和戊酸倍他米松）显示出中等效力。单纯的氟化不是抑制程度的一个因素。观察到的抑制作用与对细胞增殖或细胞更新的伴随特异性作用无关。讨论了这些发现对银屑病治疗效果以及这些类固醇常见的萎缩性副作用的可能影响。
