Eliseeva L G, Davidov E R, Gracheva I M
Biokhimiia. 1977 Jul;42(7):1195-200.
Kinetics of 14C incorporation into lipid fractions of yeast C. quilliermondii from 1-14C-octadecane and 1,2-14C-acetic acid is studied. Considerable differences, revealed in kinetics and the rate of 14C incorporation into lipid fractions depending on the lipid precursors, draw to the conclusion that the lipid synthesis from octadecane and acetic acid can occur through different pathways. The absence of the competition under the carbon influx into lipid fraction also suggests, that the lipid synthesis from ocetate and carbohydrate takes place simultaneously through two different pathways, which are spatially uncoupled in cell. A presence in yeast cells of two glyoxylate shunts is proposed, which are spatially uncoupled and specific to acetyl residues of different origins.
研究了1-¹⁴C-十八烷和1,2-¹⁴C-乙酸中¹⁴C掺入季也蒙毕赤酵母脂质组分的动力学。根据脂质前体的不同，¹⁴C掺入脂质组分的动力学和速率存在显著差异，由此得出结论：十八烷和乙酸的脂质合成可能通过不同途径进行。碳流入脂质组分时不存在竞争，这也表明乙酸和碳水化合物的脂质合成通过两种不同途径同时进行，这两种途径在细胞内空间上是不耦合的。有人提出酵母细胞中存在两种乙醛酸循环支路，它们在空间上不耦合，且对不同来源的乙酰基具有特异性。
