Ivanovska N, Philipov S
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Int J Immunopharmacol. 1996 Oct;18(10):553-61. doi: 10.1016/s0192-0561(96)00047-1.
Extracts obtained from the roots of Berberidaceae species have been used in Eastern and Bulgarian folk medicine in rheumatic and other chronic inflammatory disorders. The investigations of the chemical composition and immunological properties show that their activity is mainly due to the alkaloid constituents. In the present study the anti-inflammatory properties of total ethanol extract (TEE), three alkaloid fractions, a major alkaloid berberine and oxyacanthine isolated from Berberis vulgaris roots were compared. All these were applied in acute inflammation (carrageenan- and zymosan-induced paw oedema), as the TEE showed the highest reducing effect. Their ability to alter in vivo and in vitro complement activity was determined. Also, the TEE was most effective in a chronic inflammatory model of adjuvant arthritis. The protoberberine fractions Bv2, Bv3 and berberine suppressed a delayed type hypersensitivity (DTH) reaction. Fraction Bv1 and berberine diminished antibody response against SRBC in vivo. The in vitro treatment of splenocytes with berberine showed that the anti-SRBC antibody synthesis was influenced in a different manner depending on the time course of its application. Oxyacanthine was less effective than berberine in the tests used.
小檗科植物根提取物已被用于东方和保加利亚民间医学，治疗风湿性及其他慢性炎症性疾病。对其化学成分和免疫特性的研究表明，它们的活性主要归因于生物碱成分。在本研究中，比较了从小蘖根中分离得到的总乙醇提取物（TEE）、三个生物碱组分、主要生物碱小檗碱和刺檗碱的抗炎特性。所有这些都应用于急性炎症（角叉菜胶和酵母聚糖诱导的爪肿胀），其中TEE显示出最高的消肿效果。测定了它们在体内和体外改变补体活性的能力。此外，TEE在佐剂性关节炎的慢性炎症模型中最有效。原小檗碱组分Bv2、Bv3和小檗碱抑制迟发型超敏反应（DTH）。组分Bv1和小檗碱在体内减弱针对绵羊红细胞（SRBC）的抗体反应。用小檗碱体外处理脾细胞表明，抗SRBC抗体合成受其应用时间过程的不同影响。在所用试验中，刺檗碱的效果不如小檗碱。
