通过聚合酶链反应对口腔毛滴虫进行特异性和灵敏性检测。
Specific and sensitive detection of Trichomonas tenax by the polymerase chain reaction.
作者信息
Kikuta N, Yamamoto A, Fukura K, Goto N
机构信息
Department of Oral Microbiology, Showa University School of Dentistry, Tokyo, Japan.
出版信息
Lett Appl Microbiol. 1997 Mar;24(3):193-7. doi: 10.1046/j.1472-765x.1997.00379.x.
A polymerase chain reaction (PCR) protocol was developed for specific detection of Trichomonas tenax by using a pair of primers designed for its 18S rRNA gene. The detection was specific for T. tenax, since no amplification was detected with DNAs from Trichomonas vaginalis, which belongs to the same genus as T. tenax, in addition to various species of oral protists, fungi and bacteria, and human leukocytes. This method had a detection limit of 100 fg for T. tenax genomic DNA and could detect T. tenax cells in dental plaque at a concentration of as low as 5 cells per PCR mixture. Direct detection from clinical dental plaque samples was also possible; therefore, the present PCR procedure could provide a simple and rapid detection method of T. tenax in dental plaque.
通过使用针对其18S rRNA基因设计的一对引物，开发了一种用于特异性检测口腔毛滴虫的聚合酶链反应（PCR）方案。该检测对口腔毛滴虫具有特异性，因为除了各种口腔原生生物、真菌、细菌和人类白细胞外，来自与口腔毛滴虫同属的阴道毛滴虫的DNA未检测到扩增。该方法对口腔毛滴虫基因组DNA的检测限为100 fg，并且能够检测到每PCR混合物中低至5个细胞浓度的牙菌斑中的口腔毛滴虫细胞。也可以直接从临床牙菌斑样本中进行检测；因此，本PCR程序可为牙菌斑中口腔毛滴虫提供一种简单快速的检测方法。
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