观察性横断面研究：在智利大学牙科诊所就诊的牙周病患者中，感染阴道毛滴虫的情况。
Observational cross-sectional study of Trichomonas tenax in patients with periodontal disease attending a Chilean university dental clinic.
机构信息
Laboratory of Molecular Parasitology, Department of Medical Technology, Faculty of Health Sciences, University of Antofagasta, Angamos Avenue 601, P.O. Box 170, Antofagasta, Chile.
Biomedical Departmen, Faculty of Health Sciences, University of Antofagasta, Angamos Avenue 601, P.O. Box 170, Antofagasta, Chile.
出版信息
BMC Oral Health. 2019 Sep 4;19(1):207. doi: 10.1186/s12903-019-0885-3.
BACKGROUND
The oral flagellated protozoan Trichomonas tenax has been associated with patients with periodontal disease. However, no recent studies have been conducted on the prevalence of T. tenax in Chile. The aim of this study was to determine the presence of T. tenax in patients with periodontal disease, admitted to the Dental Clinic of the University of Antofagasta, Chile, through Polymerase Chain Reaction (PCR) amplification of the beta-tubulin gene.
METHODS
An observational, cross-sectional study was conducted on 50 patients diagnosed with periodontal disease, 20 of them with gingivitis and 30 with periodontitis. T. tenax was identified by PCR amplification of the beta-tubulin gene. Associations between the protozoan and periodontal disease or the presence of risk factors to establish T. tenax infection were determined using the chi-square test and binary logistic regression analysis.
RESULTS
T. tenax was present in 28 out of 50 (56%) of patients with periodontal disease, and was more prevalent when associated with periodontitis (21 out of 30; 70%) than dental plaque-induced gingivitis (7 out of 20; 35%). Non-statistically-significant associations were observed between the presence of T. tenax and age, gender, smoking habit or diabetes. Statistically significant associations were observed between the presence of T. tenax and periodontal disease, and between T. tenax and the Periodontal Screening and Recording (PSR) index.
CONCLUSION
T. tenax showed a high presence in patients with progressive states of periodontal diseases. Consequently, T. tenax detection is strongly recommended in patients with periodontal disease diagnosis and with a PSR index greater than 3.
背景
有鞭毛的原生动物阴道毛滴虫与牙周病患者有关。然而，最近智利没有对阴道毛滴虫的流行情况进行研究。本研究旨在通过聚合酶链反应（PCR）扩增β-微管蛋白基因，确定智利安托法加斯塔大学牙科诊所就诊的牙周病患者中是否存在阴道毛滴虫。
方法
对 50 名诊断为牙周病的患者进行了观察性、横断面研究，其中 20 名患有牙龈炎，30 名患有牙周炎。通过 PCR 扩增β-微管蛋白基因鉴定阴道毛滴虫。使用卡方检验和二元逻辑回归分析确定原生动物与牙周病之间的关联，以及确定建立阴道毛滴虫感染的危险因素。
结果
在 50 名牙周病患者中，有 28 名（56%）患者存在阴道毛滴虫，与牙周炎相关时更为常见（30 名中的 21 名；70%），而与牙菌斑引起的牙龈炎（20 名中的 7 名；35%）相比。阴道毛滴虫的存在与年龄、性别、吸烟习惯或糖尿病之间没有统计学上的显著关联。阴道毛滴虫的存在与牙周病之间以及与牙周病筛查和记录（PSR）指数之间存在统计学显著关联。
结论
阴道毛滴虫在进展性牙周病患者中存在较高的存在率。因此，建议在牙周病诊断和 PSR 指数大于 3 的患者中强烈检测阴道毛滴虫。
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