Hoja H, Marquet P, Verneuil B, Lotfi H, Pénicaut B, Lachâtre G
Department of Pharmacology and Toxicology, University Hospital, Limoges, France.
J Anal Toxicol. 1997 Mar-Apr;21(2):116-26. doi: 10.1093/jat/21.2.116.
Liquid chromatography-mass spectrometry (LC-MS), after long-term development that has introduced seven major interfacing techniques, is finally suitable for application in the field of analytical toxicology. Various compound classes can be analyzed, and sensitivities for more or less polar analytes that are as good as or better than those of gas chromatography-mass spectrometry can be obtained with modern interfaces. In addition, because ionization is often softer than classical electron impact, some LC-MS interfaces are able to handle fragile species that are otherwise not amenable to MS. This review is intended to present LC-MS to less familiarized readers and to give an extensive overview of the application of the different coupling techniques to doping agents, drugs of abuse, forensic analysis, toxic compounds of various nature, and several toxicologically relevant therapeutic drugs. Experimental parameters such as the interfaces used, ionization methods, detection limits, and experimental details for exemplary applications are given.
液相色谱-质谱联用技术（LC-MS）经过长期发展，引入了七种主要的接口技术，最终适用于分析毒理学领域。可以分析各种化合物类别，使用现代接口能够获得对极性或多或少的分析物的灵敏度，其与气相色谱-质谱联用技术相当或更佳。此外，由于离子化通常比经典电子轰击更温和，一些LC-MS接口能够处理原本不适用于质谱分析的脆弱物种。本综述旨在向不太熟悉的读者介绍LC-MS，并广泛概述不同耦合技术在兴奋剂、滥用药物、法医分析、各种性质的有毒化合物以及几种毒理学相关治疗药物方面的应用。给出了诸如所用接口、离子化方法、检测限等实验参数以及示例性应用的实验细节。
