Maurer H H
Department of Toxicology, University of Saarland, Homburg (Saar), Germany.
J Chromatogr B Biomed Sci Appl. 1998 Aug 21;713(1):3-25. doi: 10.1016/s0378-4347(97)00514-8.
This paper reviews liquid chromatographic-mass spectrometric (LC-MS) procedures for the identification and/or quantification of drugs of abuse, therapeutic drugs, poisons and/or their metabolites in biosamples (whole blood, plasma, serum, urine, cerebrospinal fluid, vitreous humor, liver or hair) of humans or animals (cattle, dog, horse, mouse, pig or rat). Papers published from 1995 to early 1997, which are relevant to clinical toxicology, forensic toxicology, doping control or drug metabolism and pharmacokinetics, were taken into consideration. They cover the following analytes: amphetamines, cocaine, lysergide (LSD), opiates, anabolics, antihypertensives, benzodiazepines, cardiac glycosides, corticosteroids, immunosuppressants, neuroleptics, non-steroidal anti-inflammatory drugs (NSAID), opioids, quaternary amines, xanthins, biogenic poisons such as aconitines, aflatoxins, amanitins and nicotine, and pesticides. LC-MS interface types, mass spectral detection modes, sample preparation procedures and chromatographic systems applied in the reviewed papers are discussed. Basic information about the biosample assayed, work-up, LC column, mobile phase, interface type, mass spectral detection mode, and validation data of each procedure is summarized in tables. Examples of typical LC-MS applications are presented.
本文综述了液相色谱-质谱联用（LC-MS）技术在鉴定和/或定量人或动物（牛、狗、马、小鼠、猪或大鼠）生物样品（全血、血浆、血清、尿液、脑脊液、玻璃体液、肝脏或毛发）中滥用药物、治疗药物、毒物和/或其代谢物方面的应用。考虑了1995年至1997年初发表的与临床毒理学、法医毒理学、兴奋剂检测或药物代谢及药代动力学相关的论文。这些论文涵盖以下分析物：苯丙胺类、可卡因、麦角酰二乙胺（LSD）、阿片类、同化激素类、抗高血压药、苯二氮䓬类、强心苷类、皮质类固醇、免疫抑制剂、抗精神病药、非甾体抗炎药（NSAID）、阿片样物质、季铵类、黄嘌呤类、生物毒素如乌头碱、黄曲霉毒素、鹅膏毒素和尼古丁，以及农药。讨论了综述论文中应用的LC-MS接口类型、质谱检测模式、样品制备程序和色谱系统。各程序的生物样品分析、预处理、LC柱、流动相、接口类型、质谱检测模式及验证数据的基本信息在表格中进行了总结。文中还给出了典型LC-MS应用的实例。
